A Differential Interference Imaging System That Can Change Shearing Direction and Size Rapidly

Abstract

The invention relates to a differential interference imaging system capable of rapidly changing the shearing direction and size, including: a light source, an optical filter, a polarizer, a sample stage, an infinite-distance imaging microscope objective lens, a lens tube lens, a shearing assembly, a detector polarizer, image sensor. The linearly polarized light emitted by the light source is adjusted by the optical filter to adjust the light intensity and the polarizer to adjust the polarization direction, then passes through the transparent sample placed on the sample stage, is collected by the infinity imaging microscope objective lens, and is imaged by the lens tube lens, the imaging beam The sheared component is divided into two beams of linearly polarized light fields whose polarization directions are perpendicular to each other and with a small shear amount, and then synthesized by the analyzer to form an interference light field, and finally a differential interference image is formed in the image sensor. The differential interference imaging system provided by the invention can be flexibly assembled on a conventional optical microscope, has a simple structure and is easy to implement, and can study the morphology and structure of a sample by performing high-quality quantitative phase measurement on a non-stained sample.

Description

technical field [0001] The invention relates to the field of optical technology, in particular to a differential interference imaging system capable of rapidly changing the shearing direction and size. Background technique [0002] Differential interference imaging system is widely used in the detection and imaging of transparent samples. Such as differential interference phase contrast microscope, it mainly cuts the interference imaging beam at a certain distance in the transverse direction, and converts the optical path difference of the two beams in different characteristic areas of the sample into intensity changes, producing a visual pseudo 3D (relief) effect. Therefore, the details of the transparent sample can be clearly observed. Compared with other types of microscopy techniques, it has the advantages of outstanding phase contrast, high spatial resolution and optical sectioning ability, and does not require fluorescent labeling and staining, so that in-situ observa...

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Problems solved by technology

[0003] Traditional differential interference imaging systems usually use Nomarski prisms to cut the object beam along the lateral direction. This method has some obvious disadvantages: first, special prisms are required, and the processing and debugging of prisms are quite complicated; second, using differential interference When the imaging system observes the sample, due to the different properties of the sample and the replacement of different magnification objective lenses, the shear amount needs to be changed to achieve a better observation effect. The traditional method is to replace different Normarski prisms, which increases the complexity of the operation; The third is that the shearing direction of the shearing prism is not easy to adjust, which greatly limits the use; the fourth is that the quantitative measurement of the sample cannot be realized, and it is difficult to meet the needs of data analysis.

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