Kit for detecting homocysteine

Abstract

The invention relates to the technical field of medical detection, in particular to a kit for detecting homocysteine. The kit for detecting homocysteine comprises a shell, a reagent and an informationcard, wherein a standard curve of absorbance values to concentration values is stored in the information card, a correction factor is stored in the information card, and the correction factor is usedfor correcting the concentration of homocysteine in a blood sample when the to-be-detected blood sample is whole blood; and the reagent comprises a treatment solution, a first reaction solution and asecond reaction solution, the treatment solution is used for converting protein-binding homocysteine in the blood sample into free homocysteine, and the first reaction solution and the second reaction solution are used for reacting with the free homocysteine. When the kit for detecting the homocysteine is in use, an analyzer can be used for directly obtaining the concentration of the homocysteineaccording to the absorbance value-to-concentration value standard curve which is stored in the information card, so that the kit is very convenient. In addition, the concentration of the homocysteinein the whole blood sample can be corrected through the correction factor.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of medical detection, in particular to a kit for detecting homocysteine. Background technique [0002] Homocysteine ​​(HCY) is an important risk factor for atherosclerotic vascular diseases, and it is an intermediate product formed by the metabolism of methionine. Elevated homocysteine ​​(HCY) concentration in blood (concentration ≥ 12umol / L) is a risk factor for coronary heart disease, stroke, peripheral vascular atherosclerosis, and arteriovenous embolism. Elevated homocysteine ​​(HCY) in blood High levels increase the morbidity and mortality of cardiovascular diseases, so it is very important to determine the concentration of homocysteine ​​(HCY) in blood. [0003] At present, the methods for determining homocysteine ​​(HCY) mainly include the following: cyclic enzymatic technique; high performance liquid chromatography (HPLC), enzyme immunoassay (EIA), ion chromatography, capil...

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Problems solved by technology

[0004] However, in the process of realizing the embodiments of the present invention, the inventors of the present invention have found that the cystathionine cycle enzymatic method mainly converts cystathionine (HCY) into β-cystathionine by measuring homocysteine ​​(HCY). Determine the content of homocysteine ​​(HCY), but cannot eliminate the interference of cystathionine carried by the patient itself; the hydrolase cycle enzyme method has high specificity and strong anti-interference ability, but the current test reagents are low in sensitivity and complicated to operate

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