An indel marker, detection primer set and development method associated with growth traits of Pinctada martensii
A technology for growth traits of Pinctada martensii, applied in biochemical equipment and methods, microbial measurement/inspection, bioinformatics, etc., can solve the problems of not being fully utilized and achieve the effect of reducing workload
Active Publication Date: 2022-02-11
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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[0004] In addition, there are still a large number of studies that screen differentially expressed genes through transcriptome sequencing, and analyze the molecular genetic basis of trait formation based on gene expression, but have not yet found that SNPs or InDels in differential genes are fully utilized and associated with traits. report
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[0033]100 individuals were randomly selected from the Shenzhen population of Pinctada martensii, and the attachments on the shells were cleaned. Measure and record the shell length, height, width and body weight of each individual one by one. Then sort the 100 individuals by size according to the shape of the hinge line, take 20% of the individuals ranked before and after each as samples, the top 20% of individuals as the maximum value group (L), and the last 20% of individuals as the minimum value group(s). The mantle tissue was dissected and stored in liquid nitrogen for RNA extraction.
[0034] The fixed tissues of 3 individuals were taken from the L and S groups respectively, the total RNA was extracted, and the transcriptome was sequenced and assembled. Align CleanReads with the reference genome to obtain position information on the reference genome or gene.
[0035] The Pearson Correlation Coefficient r (Pearson Correlation Coefficient) was used as the evaluation inde...
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The invention discloses an InDel marker associated with the growth traits of Pinctada martensii, a detection primer set and a development method. The InDel marker is located in the insertion or deletion sequence TNNN between the 103rd and 104th bases from the 5' end of the sequence shown in SEQ ID NO.1, where NNN represents the unknown base and the number of bases. Using the development method of the present invention to creatively screen InDels from differentially expressed genes and associate them with traits, a molecular marker SNP441 associated with traits is developed. The detection primer set designed by using the molecular marker SNP441 can be used for genotyping of the molecular marker SNP441 of Pinctada martensii to be tested, and the individuals with growth advantages can be screened out to be used as backup parents for breeding, so as to realize molecular marker-assisted breeding.
Description
Technical field: [0001] The invention belongs to the technical field of aquatic animal genetics and molecular marker assisted breeding, and specifically relates to an InDel marker associated with the growth traits of Pinctada martensii, a detection primer set and a development method. Background technique: [0002] Pinctada martensii is the main species of pearl culture in my country and the world. Since the successful artificial breeding of Pinctada martensii in my country in 1965, the seawater pearl industry has developed rapidly and has become one of the pillar industries of mariculture in Guangdong, Guangxi and some coastal areas of Hainan. In the past 10 years, the quality and output of seawater pearls in my country have declined significantly, the international competitiveness has weakened, and the economic benefits of breeding have declined. The reason, in addition to the deterioration of the breeding environment and the backward breeding technology, is another impor...
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IPC IPC(8): C12Q1/6888C12Q1/6858C12Q1/6809C12N15/11G16B20/20G16B25/20G16B25/10G16B35/20
CPCC12Q1/6888C12Q1/6858C12Q1/6809G16B20/20G16B25/20G16B25/10G16B35/20C12Q2600/124C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 何毛贤张华刘文广石禹
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI