Preparation method and application of pulmonary artery tissue unicellular suspension

A single-cell suspension, pulmonary artery technology, applied in the field of biomedicine, can solve the problems of incompatibility, the single-cell suspension is not easy to transport, and the cell viability cannot be guaranteed, and achieves the effect of easy operation and good practical application value.

Active Publication Date: 2020-09-04
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

The current implementation method is to send the single-cell separation equipment to the hospital, and the fresh tissue obtained by the operation is immediately digested and subsequently separated, which greatly limits the cost, time, and place of the experiment.
Moreover, single-cell sequencing experiments require a large number of isolated cells with cell ...

Method used

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  • Preparation method and application of pulmonary artery tissue unicellular suspension
  • Preparation method and application of pulmonary artery tissue unicellular suspension
  • Preparation method and application of pulmonary artery tissue unicellular suspension

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Embodiment Construction

[0034] Below in conjunction with embodiment, further set forth the present invention.

[0035] Obtaining and processing tissue samples:

[0036] Pulmonary artery endarterectomy tissue samples were obtained during the operation ( figure 1 A). The pulmonary artery endarterial stripping tissue specimens obtained during the operation were selected for separation, cleaning, and trimming ( figure 1 B), select the light yellow tissue components of the inner membrane to prepare the single cell suspension, and remove the blood thrombus components in the exfoliated tissue as much as possible, as well as the muscularized inner membrane tissue, because this part of the tissue contains almost no cellular components, which will affect the subsequent single cell suspension. The efficiency of liquid preparation is not conducive to the preparation of single cell suspension. The light yellow tissue components of the inner membrane used for the preparation of single cell suspension treated wi...

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Abstract

The invention provides a preparation method of a pulmonary artery tissue unicellular suspension for unicellular sequencing. The unicellular suspension can be obtained by separating, cleaning, trimming, incubating and enzymatically digesting pulmonary endarterectomy tissues. According to the method disclosed by the invention, while the cell quality is ensured, a large number of separated cells derived from the pulmonary endarterectomy tissues can be obtained at a time; and a used reagent is simple and convenient to operate, and the method has good practical application value.

Description

technical field [0001] The invention provides a method for preparing a single-cell suspension of pulmonary artery endarterectomy tissue used for single-cell sequencing, which belongs to the field of biomedical technology, and specifically relates to a method for preparing a single-cell suspension of pulmonary artery endarterectomy tissue for single-cell sequencing Preparation method and application. Background technique [0002] Chronic thromboembolic pulmonary hypertension (CTEPH) is a disease characterized by dyspnea, fatigue, and reduced exercise tolerance due to unresolved thrombus emboli occluding the proximal pulmonary artery with accompanying distal pulmonary vascular Remodeling, leading to increased pulmonary vascular resistance, progressive increase in pulmonary artery pressure and progressive increase in right heart load, eventually progressing to right heart failure, is a serious, progressive, and fatal disease. The incidence of CTEPH is increasing year by year. ...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12Q1/6806
CPCC12N5/0688C12N2509/00C12Q1/6806C12Q1/6869
Inventor 缪冉杨媛华钟久昌翟振国董星琲龚娟妮李一丹王剑锋万钧郭晓娟王莹杨苏乔李积凤邝土光
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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