Application of composition in prevention or treatment of diseases caused by escherichia coli of broiler chickens
A technology of composition and use, applied in the field of medicine, can solve problems to be studied, achieve good treatment, increase lipase activity, and increase the effect of quantity
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[0033] Determination of the diameter of the inhibition zone
[0034] The diameter of the inhibition zone of the composition against Escherichia coli O78 was measured by a plate agar punching method. Take 0.1mL concentration as 10 8 Place the CFU / mL bacterial solution on the prepared sterile MacConkey culture plate, spread evenly, stop coating the plate when the spreader and the plate have a certain viscosity, and punch holes on the plate with a puncher with a diameter of 6 mm , fill the well with the composition solution and physiological saline with a concentration of 1 g / mL. Place the plate upright in a constant temperature incubator at 37°C, take out the plate after 24 hours of incubation, and measure the diameter of the inhibition zone with a vernier caliper.
[0035] Determination of minimum inhibitory concentration
[0036]The minimum inhibitory concentration of the composition against avian pathogenic Escherichia coli O78 was determined by the test tube double diluti...
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