57 results about "Escherichia coli infection" patented technology

The invention relates to an echinacea extract and a preparation method of the echinacea extract. The echinacea extract provided by the invention is obtained through carrying out twice extraction on echinacea medicinal materials by alkaline ethanol solution and lysozyme; and in the product, the polysaccharide content is greater than or equal to 21.50 percent, the polyphenol content is greater than or equal to 8.10 percent, and the chicoric acid content is greater than equal to 5.00 percent. Because the polyphenol content and the chicoric acid content of the echinacea extract are obviously increased, the echinacea extract is prepared into ordinary preparations including powder or oral liquid and the like, and the curative effect is greatly improved. In a compound preparation processed by the echinacea extract and bacteriolysant according to a consumption ratio being 3:1, active ingredients of echinacea and lysozyme totally account for 20 percent, wherein the consumption ratio of the echinacea extract to the lysozyme is (4-1):1, auxiliary materials of cane sugar account for 64 percent, and dextrin accounts for 16 percent. The compound preparation has the functions of improving the immunity of live stock and realizing the sterilization and antivirus functions, and can be used as medicine for preventing and treating animal diseases, particularly chicken diseases caused by escherichia coli infection.

The invention relates to a compound composition containing plumbago zeylanica for treating livestock and poultry escherichia coli infection diseases. The compound composition is prepared from the following materials in parts by weight: 3-30 parts of antibacterial drug, and 5-200 parts of plumbago zeylanica. The materials are prepared into powder, tablets, oral liquid or granules after being evenly mixed, and the compound composition is added to livestock and poultry feeds to feed according to the feed adding amount of 0.1-7 g/kg; and the tolerance of bacteria on the antibacterial drug can be changed over. Thus, the treatment effect of the antibacterial drug is improved; the drug cost is greatly reduced; and higher economic benefits are created for farmers.

The present invention relates to a composition comprising the bacteriophage EK88P-1 isolated from the nature and having a broad antibacterial spectrum against E. coli as an active ingredient, and a method for preventing and treating E. coli infections using the said composition. The bacteriophage EK88P-1, the active ingredient of the composition of the present invention, has a broad antibacterial spectrum against E. coli and has the genome characteristically composed of the partial nucleotide sequences represented by SEQ. ID. NO: 1˜NO: 25, and also characterized by the bacteriophage belonging to the Myoviridae family according to the morphology that is composed of the major structural proteins in the sizes of approximately 49 kDa, 53 kDa, 94 kDa, and 103 kDa.

The present invention discloses a nitrogen-heterocycle-containing artemisinin derivative and a preparation method thereof, the derivative is a compound of a formula I, and X is imidazolyl, thiazolyl, oxazolyl or thiazolidinyl. The present invention also discloses use of the derivative in resisting Escherichia coli infection, and especially, the derivative shows a very strong synergistic antibacterial effect on Escherichia coli by combination with an artemisinin drug.

The invention discloses a fermented Chinese herbal preparation capable of preventing escherichia coli infection in broiler chickens. The active ingredient of the fermented Chinese herbal preparation is a culture obtained by fermenting the following raw material in a formula by virtue of lactobacillus in percentage by mass: 77.7-97.7% of a composite Chinese herbal medicine, 0-20% of bran, 1.3% of ammonium sulfate and 1.0% of glucose, wherein the composite Chinese herbal medicine is prepared by mixing detoxified powder of ageratina adenophora herb, bighead atractylodes rhizome, poria cocos, bunge corydalis herb, cortex phellodendri and curcuma zedoary at the mass ratio of 5:1:1:1:1:1. Furthermore, the invention also discloses a preparation method of the fermented Chinese herbal preparation. The fermented Chinese herbal preparation prepared by the invention can be used for preventing and treating escherichia coli-induced diarrhea in broiler chickens through the combination of probiotics and the Chinese herbal medicine and replacing the efficacy of antibiotic, and the fermented Chinese herbal preparation can be also used for developing the probiotic effect as a micro-ecological preparation; and the fermented Chinese herbal preparation is pure natural, free from toxicity and harm, free from drug residue, free from foreign smell, good in palatability, and safe and convenient to use.

The invention relates to a breeding method of a new Berkshire pig disease-resistant variety. The breeding method comprises the steps that firstly, weaned piglets of a Berkshire pig core group are subjected to Escherichia coli infection experiments by adopting a feed adding method, according to a phenotype identification result, a Berkshire pig Escherichia coli disease-resistant breeding base group is established; secondly, the base group is subjected to molecule breeding with general resistance to diseases by using a proprietary technology obtained in the early stage, meanwhile characters such as breeding performance, growing speed, lean meat percentage and carcass quality are detected and selected, and a first generation is built; thirdly, a group continuous progeny breeding and molecular marker assisted selection method is adopted, after four to five generations, an established breeding aim is achieved step by step, and a new variety core group is established. The new Berkshire pig disease-resistant variety has the advantages of resistance to weaned piglet diarrheal diseases, high stress resistance, high environmental adaptability and the like, and can further provide guidance for disease-resistant breeding and new variety selection and breeding of other pig varieties.

The invention relates to a compound composition for treating a livestock escherichia coli infection disease. The compound composition is prepared from the following crude drugs in parts by weight: 3-30 parts of antibacterial drug, and 5-200 parts of phryma leptostachya. The raw materials are prepared into powder, pills, oral liquid or granules after being evenly mixed, and 0.1-7g/kg of feed is added to the livestock and poultry feed to feed, so that the drug resistance of germs on the antibacterial drug can be reversed, so as to improve the treatment effect of the antibacterial drug, and greatly reduce the medication cost and the drug residue. Higher economic benefits are created for farmers, and the food safety for men is ensured.

The invention provides a compound coneflower preparation, wherein a use ratio of effective components such as a coneflower extract and lysozyme is 3:1, the total content of the coneflower extract and the lysozyme is 25%, and contents of auxiliary materials such as sucrose and dextrin are respectively 60% and 15%. According to the present invention, the selected coneflower extract is obtained by extracting a coneflower herb with an alkaline ethanol solution and lysozyme; the polysaccharide content exceeds 20%, the polyphenol content exceeds 8%, and the chicoric acid content exceeds 5%; and the composite preparation has functions of animal immunity improving, anti-bacterial and anti-virus, and can be used for preparation of drugs for prevention and treatment of animal diseases, especially prevention and treatment of chicken diseases caused by escherichia coli infection.

The invention discloses a compound echinacea purpurea preparation. The dose ratio of an echinacea purpurea extract as an effective component to lysozyme is (4-1):1, the dosage of the echinacea purpurea extract and the lysozyme is 20% in total, and the ajudvants contains 64% of saccharose and 16% of dextrin. The echinacea purpurea extract selected by the invention is obtained by extracting echinacea purpurea medicinal material by alkaline ethanol solution and lysozyme for two times, wherein the content of polysaccharide is not less than 21.5%, the content of polyphenol is not less than 8.10% and the content of chicoric acid is not less than 5.00%. The compound preparation provided by the invention has the effects of improving animal immunity, antibiosis and anti-virus, thereby being capable of being taken as the medicine for preventing and treating the animal diseases, in particular to the chicken diseases caused by escherichia coli infections.

The invention discloses compound neomycin sulfate oral liquid for treating severe diarrhea of poultry. The oral liquid comprises the following components in parts by weight: 8 to 15 parts of neomycin sulfate, 3 to 6 parts of berberine hydrochloride, 1 to 3 parts of lactate TMP, 10 to 20 parts of injection astragalus polysaccharide, 2 to 4 parts of levamisole hydrochloride, 5 to 15 parts of acetaminophen, and 60 to 80 parts of injection water. The compound neomycin sulfate oral liquid for treating the severe diarrhea of the poultry is applied to the poultry, and is used for Gram-negative bacteria-induced gastrointestinal severe infection of the poultry and used for treating enteritis and diarrhea of the poultry. By using the oral liquid for treating escherichia coli infection and unknown severe diarrhea of the poultry, the significant efficiency is 94.1 percent, and the effective rate is 99.8 percent. The oral liquid is reasonable in formula compatibility, high in defecation level, low in dust content, low in pollution, high in oral bioavailability and convenient for industrialization.

The invention discloses a gene for encoding recombined swine IL 22. The invention further discloses a recombined expression carrier containing a recombined gene, a transgene cell system, a transgene recombined bacterial and a host cell. The invention further discloses a recombined swine IL 22 and its extraction and purification method. The invention further discloses an application of recombined plasmid pET-32a(+)-PIL-22, recombined strain and recombined protein in anti-apoptosis and prevention of escherichia coli infection. The carrier pET-32a(+) of the expression recombined swine IL 22 structured in the invention contains His label, the recombined swine IL 22 protein extracted from escherichia coli is connected with the His label; through a His pillar, the highly purified recombined swine IL 22 protein is acquired; the expression output of the recombined swine IL 22 protein is counted through Quantity One software, the purity is up to 95.7%.

The invention relates to a traditional Chinese medicine composition preventing and treating escherichia coli infection in the brooding stage of poultry. According to the dialectical characteristics of escherichia coli infection in the brooding stage of poultry, ophiopogon japonicus for nourishing yin and generating body fluid and rehmannia roots for cooling blood and nourishing yin jointly serve as monarch medicine, radix berberidis for purging intense heat and removing toxic matter serves as minister medicine, and astragalus for benefiting qi and strengthening superficies, atractylodes for strengthening the spleen and benefiting qi and licorice for tonifying the spleen and benefiting qi together serve as assisting and guiding medicine. On one hand, the cold properties of the monarch and minister medicine are relieved, and on the other hand, the medicine has the functions of consolidating the basis and can meet the requirement of effectively preventing and controlling escherichia coli infection on the premise that the internal organs of young poultry are immature in the growth and development stage of the young poultry. As shown by a larger amount of clinical application to poultry diseases, the traditional Chinese medicine composition has the advantages that the effect is exact, toxic and side effects are avoided, and medicine residuals are avoided.

The invention provides a compound plant extract for relieving diarrhea of weaned piglets and improving intestinal health of the weaned piglets. The extract is prepared from the following components inparts by mass: 30-35 parts of flos lonicerae extract, 30-35 parts of fructus forsythiae extract, 10-20 parts of radix scutellariae extract and 20-30 parts of rhizoma coptidis extract. In combinationwith the research progress of the efficacy of plant extracts and the experience of application of the plant extracts to pig production, the compound plant extract product (PEM) is prepared from the plant extracts prepared from the selected flos lonicerae extract, fructus forsythiae extract, radix scutellariae extract and rhizoma coptidis extract in a certain ratio. The pig feeding experiment proves that with addition of the compound plant extract product to the diet, diarrhea of the piglets can be effectively reduced, the growth performance of the piglets is improved, and immune stress causedby escherichia coli infection is relieved.

The invention belongs to the technical field of veterinary biology, and in particular relates to a yolk antibody for preventing and treating colibacillosis and a preparation method and application of the yolk antibody. The escherichia coli yolk antibody is prepared by using a mixed solution of extracted avian pathogenic escherichia coli O78 outer membrane protein and pilin as antigen immune chicken; and experiments show that the yolk antibody is safe and efficient and has a good protective effect on homogenous and multiple alien escherichia coli. The yolk antibody can be used for preventing and treating multi-serotype colibacillosis, and can also be used as an additive of health-care products, food and feed or used for preparing preparations for detecting related diseases caused by escherichia coli infection.

The present invention discloses a granule for treatment of heat toxin symptom caused by chicken escherichia coli infection. The raw material ratio by crude medicines includes: 30-90g of gypsum, 3-20g of honeysuckle, 2-30g of figwort root, 2-15g of baical skullcap root, 2-15g of dried radix rehmannia, 2-15g of fructus forsythiae, 2-15g of fruit of cape jasmine, 2-15g of gentiana scabra bunge, 2-30g of bunge corydalis herb, 2-40g of isatis root, 2-15g of rhizoma anemarrhena, and 2-15g of radix ophiopogonis. The granule also includes an excipient. The preparation method of the granule includes: (1) extracting the prescription medicine to make extract powder; (2) adding the excipient and mixing; and (3) granulating, drying and packaging to get finished products. The granule of the present invention has a trade name of clearing and releasing granule, has the effect of clearing heat and detoxifying, is significant in effects and easy to take, and has no toxic side effects.

The invention relates to a process for preparing high purity raw powder of camellia seed triterpenoid saponin additive of livestock and poultry nourishing products, which belongs to the technical field of the process for preparing the livestock and poultry nourishing products. The invention aims to solve the technical problems of providing the process for preparing the high purity raw powder of the camellia seed triterpenoid saponin additive of the livestock and poultry nourishing products. The technical schemes which solves the technical problems are that the invention comprises the steps of the preparation of degreasing camellia seed power, the preparation of camellia seed triterpenoid saponin additive extract, the preparation of camellia seed triterpenoid saponin additive concentrated extract, the preparation of camellia seed triterpenoid saponin additive tobacco extract, column chromatography and drying. The invention is used to prepare the raw powder of camellia seed triterpenoid saponin additive of livestock and poultry nourishing products and is used as an addition agent of livestock and poultry feeds and drugs, the livestock and poultry nourishing products which contain antibiotics are replaced, and the purity of the raw powder is high. The beneficial effects are that the invention is capable of inhibiting the growth of bacillus coli and salmonella, is capable of treating diseases such as yellow scour of newborn piglets, white diarrhea, white diarrhea, typhoid fever, escherichia coli infection and salpingitis and the like, thereby adding the survival rate of piglets and the daily increase, and reducing the diarrhea rate.

The invention belongs to the technical field of veterinary medicines, and concretely relates to a traditional Chinese veterinary medicinal compound composition for preventing and treating serious Escherichia coli infection of livestock and poultry, and a preparation method thereof. The traditional Chinese veterinary medicinal compound composition for preventing and treating serious Escherichia coli infection of livestock and poultry comprises poplar flower, chlorogenic acid, natural indigo, Amur Corktree Bark, Agrimonia pilosa Ledeb., Meadowrue Root and Rhizome, Weeping Forsythia and purple daisy. The traditional Chinese veterinary medicinal compound composition has no medicine resistance problem, is safe and environmentally-friendly, has the efficacy of clearing heat, detoxifying and preventing bacteria and toxins, and is especially suitable for treating livestock and poultry enteritis, dysentery and other intestinal diseases induced by serious Escherichia coli infection, and the preparation method is simple and reduces the medication cost of the animal husbandry production.

The invention relates to a method for treating animal escherichia coli infected diseases. The method comprises the following steps: mixing phryma leptostachya and an antibacterial medicament for treating animal escherichia coli infection at a weight ratio of (3-30):(5-200) to obtain a compound medicament containing the phryma leptostachya, adding the compound medicament into an animal feed according to the adding quantity of 0.1-7g/kg feed, and continuously feeding animals for 3-7 days. By adopting the method, the tolerance of bacteria to the antibacterial medicament can be effectively reversed, so that the treatment effect of the antibacterial medicament is improved, the medicament cost and the medicament residue are greatly reduced, high economic benefits are created for farmers, and the food safety of human beings is guaranteed.

The invention discloses a goat F17 escherichia coli infection resisting gene screening method which includes the steps: F17 escherichia coli infection diseased lamb and disease-resistant lamb screening and spleen and intestinal canal content sample acquisition; F17 escherichia coli infection diseased lamb and disease-resistant lamb spleen sample high-throughput sequencing; F17 escherichia coli infection diseased lamb and disease-resistant lamb spleen transcriptomics comparative analysis; F17 escherichia coli infection resisting gene (circRNA) screening and identifying. The goat F17 escherichiacoli infection resisting gene screening method is used for screening F17 escherichia coli infection resisting genes (circRNA) from disease-resistant lamb spleens in a targeted manner, is high in accuracy and pertinence and saves time.

The invention relates to a method for treating Escherichia coli infection diseases by using creat and antibacterial agents, which is characterized in that the creat and antibacterial agents for treating Escherichia coli infection are mixed in a weight ratio of (5-200):(3-30) to obtain a creat-containing compound medicine; and 0.1-7g of the compound medicine is added to every kilo of feed for livestock and fowl for continuous feeding for 3-7 days. The antibacterial agents for treating Escherichia coli infection are beta-lactam antibacterial agents. The compound medicine can reverse the drug resistance of bacteria to antibacterial agents, thereby enhancing the treatment effect of the antibacterial agents, greatly lowering the medicine cost and medicine residue, creating higher economic benefit for farmers and ensuring the human food safety.

The invention relates to an IncRNA marker related to bacterial diarrhea resistance of piglets and application of the IncRNA marker. According to the IncRNA marker and the application thereof, a key candidate lncRNA-FUT3-AS1 for Escherichia coli F18 infection regulation is screened through omics sequencing, the sequence is SEQ ID NO: 1, and on the basis, deep analysis is carried out around the function and regulation mechanism of the FUT3-AS1 in the research; firstly, qPCR and Northern blot technologies are used for detecting the expression difference condition of the FUT3-AS1 in duodenums of F18 Escherichia coli sensitive and resistant weaned piglets of Meishan pigs and Sutai pigs, lncRNA-FUT3-AS1-silenced pig small intestine epithelial cells are successfully established, and the interference efficiency reaches 68%; and a series of detection means such as bacterial counting, Gram's staining, scanning electron microscopy and indirect immunofluorescence find that the adhesion capability of F18 Escherichia coli to IPEC-J2 cells can be remarkably lowered by silencing lncRNA-FUT3-AS1. Therefore, the lncRNA-FUT3-AS1 plays an important resistance regulation function in a process of resisting F18 Escherichia coli infection of the weaned piglets, the adhesion ability of the small intestine epithelial cells to the F18 Escherichia coli is lowered by down-regulating an expression level of the lncRNA-FUT3-AS1, and the resistance ability of the piglets to the F18 Escherichia coli infection is favorably improved.