Application of antibacterial peptide in in-vitro inhibition of Escherichia coli

Abstract

The invention belongs to the field of cell biology, and particularly relates to an application of an antibacterial peptide in the in-vitro inhibition of Escherichia coli. The antibacterial peptide selected by the invention is a natural antibacterial peptide Esculentin-1A, and the amino acid sequence information of the antibacterial peptide Esculentin-1A is shown as SEQ ID NO.1. In the research process, it is found that the antibacterial peptide Esculentin-1A and citric acid are mixed according to a mass ratio of (7-10):(2-5), and in-vitro tests prove that the inhibition of the mixture on Escherichia coli reaches 99% or more. The antibacterial peptide and citric acid are mixed for use for the first time, and a new research and development direction is provided for the development of drugs related to the treatment of symptoms such as cholecystitis and ovaritis caused by Escherichia coli infection.

Description

technical field [0001] The invention belongs to the field of cell biology, and in particular relates to the application of an antimicrobial peptide in inhibiting Escherichia coli in vitro. Background technique [0002] Antimicrobial peptide refers to a class of basic polypeptide substances with antibacterial activity induced by insects, with a molecular weight of about 2000-7000 and composed of 20-60 amino acid residues. Most of these active peptides have the characteristics of strong alkalinity, thermal stability and broad-spectrum antibacterial properties. It can be seen from the published literature on antimicrobial peptides that, in addition to the different sources of classification, according to their structure and characteristics, the following three classification methods are common. One is to divide antimicrobial peptides into five categories: cecropins, magainins, defensins, tachyplesins and endogenous cathelicidins. According to the secondary structure and confo...

AI Technical Summary

Problems solved by technology

However, the function of the currently known synthetic antimicrobial peptides is mainly to inhibit Gram-negative bacteria and fungi, and their bactericidal effect is not good

[0005] Chinese patent CN1629837C discloses a group of synthetic antimicrobial peptides, which have stronger bactericidal activity than natural antimicrobial peptides, and can be used to prepare drugs for treating diseases caused by Gram-negative bacteria, Gram-positive bacteria and fungal infections ; This antimicrobial peptide is only suitable for in vivo sterilization, and the bactericidal effect is average, and it cannot achieve the purpose of strong sterilization in vitro

[0006] To sum up, the ubiquitous defect in the prior art is that the bactericidal effect is average, and most of them are sterilized in vivo, which cannot meet the needs of in vitro sterilization.

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