Rapid quantitative fluorescence detection device for 3-deoxyfructose and preparation method thereof

A deoxyfructose, quantitative detection technology, applied in measurement devices, biological tests, material inspection products, etc., can solve the problem that the concentration of 3-deoxyfructose cannot be detected, the fluorescence is easily quenched, and the NC membrane has insufficient protein adsorption and weak binding force. and other problems, to achieve the effect of improving reasonable comprehensive judgment, increasing complexity and strong specificity

Active Publication Date: 2020-09-08
JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention provides a 3-deoxyfructose rapid quantitative detection device and its preparation method to solve the problem that the concentration detection of 3-deoxyfructose cannot be realized without borrowing a large instrument, and the fluorescence is easily quenched and the amount of protein adsorbed by NC membrane in the prior art Insufficient and weak combination

Method used

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  • Rapid quantitative fluorescence detection device for 3-deoxyfructose and preparation method thereof
  • Rapid quantitative fluorescence detection device for 3-deoxyfructose and preparation method thereof
  • Rapid quantitative fluorescence detection device for 3-deoxyfructose and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0037] The sample pad 1, the immunofluorescent antibody glass fiber membrane 2, the immunonitrocellulose membrane 3, and the absorbent pad 4 are respectively pasted on the plastic plate 5, and the two ends of the immunonitrocellulose membrane 3 are respectively connected to the absorbent pad 4, the immunofluorescent antibody glass The fiber membrane 2 is overlapped, and the other end of the immunofluorescence antibody glass fiber membrane 2 is overlapped with the sample pad 1; a detection line T and a quality control line C are set on the immunonitrocellulose membrane 3, and the detection line T The upper solid phase has 3 deoxyfructose-carrier protein; or the first detection line T line solid phase 3 deoxyfructose-carrier protein, and the quality control line C is sprayed with goat anti-rabbit IgG polyclonal antibody; There are 3 deoxyfructose-carrier proteins on the solid phase of the detection line T, and the 3-deoxyfructose-carrier proteins refer to: ovalbumin OVA, or bovin...

Embodiment 2

[0057] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin and polylysine (SIGMA, 150KD), wherein the concentration of polyethylene glycol glycerin is 0.5%, and polylysine The concentration is 0.5%, filtered through a 0.22μm filter membrane, and set aside;

[0058] Sample pad pretreatment

[0059]Soak the glass fiber in the sample pad treatment solution for 10 minutes, the sample pad treatment solution includes: Tris-HCL solution concentration 0.1M, bovine serum albumin BSA concentration 0.7%, casein concentration 0.15%, surfactant alkyl The concentration of phenol polyoxyethylene ether is 0.7%, and it is dried at 37°C for later use. After the treatment, the sample pad can improve the reaction sensitivity;

[0060] All the other are with embodiment 1.

Embodiment 3

[0062] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin, polylysine (SIGMA, 150KD) and PEG2000, wherein the concentration of polyethylene glycol glycerin is 0.5%, poly The concentration of lysine is 0.5%, the concentration of PEG20000 is 0.1%, and it is filtered through a 0.22 μm filter membrane, and it is set aside;

[0063] Sample pad pretreatment

[0064] Soak the glass fiber in the sample pad treatment solution for 10 minutes, the sample pad treatment solution includes: Tris-HCL solution concentration of 0.1M, bovine serum albumin BSA concentration of 1%, casein concentration of 0.2%, surfactant alkyl The concentration of phenol polyoxyethylene ether is 1%, and it is dried at 37°C for later use. After the treatment, the sample pad can improve the reaction sensitivity;

[0065] All the other are with embodiment 1.

[0066] Further illustrate the effect of the present invention by experiment below.

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Abstract

The invention relates to a rapid quantitative fluorescence detection device for 3-deoxyfructose and a preparation method thereof, and belongs to the field of medical detection. The device is preparedby adhering a nitrocellulose membrane with a solid phase containing 3-deoxyfructose-carrier protein and a goat-anti-mouse IgG polyclonal antibody, glass fiber adsorbed with an immunofluorescence microsphere labeled 3-deoxyfructose antibody, a sample pad, absorbent paper and other auxiliary materials. The preparation method comprises the following steps: pretreating a nitrocellulose membrane by adopting a polyethylene glycol glycerol treatment solution; firstly combining carrier protein-3-deoxyfructose with oleic acid modified zinc sulfide nanoparticles, and then adsorbing onto a nitrocellulosemembrane; and preparing the proper buffer solution and the sample pad treatment solution. The reaction sensitivity is effectively improved on the basis of ensuring complete release of the immunofluorescence microsphere, and under the same threshold value, the dosage of the immunofluorescence microsphere can be reduced, the cost is saved, and the complexity of production operation is not increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.

Description

technical field [0001] The invention relates to the field of medical detection equipment, in particular to a rapid quantitative detection device for 3-deoxyfructose and a preparation method thereof, which uses immunofluorescence chromatography technology and the principle of competition method to quantitatively detect 3-deoxyfructose in whole blood, serum, plasma or urine samples The content detection device and the preparation method thereof can realize rapid detection of 3-deoxyfructose, and have high sensitivity and strong specificity. Background technique [0002] The incidence of hyperglycemia in my country is increasing year by year, and the population of diabetic patients is expanding year by year; diabetic complications, such as diabetic cardiovascular disease, diabetic foot, diabetic nephropathy, diabetic eye disease, etc., have caused great damage and economic burden to human beings; how to solve these complications It has become a hot spot of concern and research i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/66G01N33/58G01N33/558G01N33/543
CPCG01N33/66G01N33/582G01N33/558G01N33/54346G01N2800/042
Inventor 杨小军李欣
Owner JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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