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Aspergillus flavus tl-f3 strengthens the method of ryegrass to remediate heavy metal polluted soil

A TL-F3, polluted soil technology, applied in the field of Aspergillus flavus TL-F3 strengthening ryegrass to repair heavy metal polluted soil, can solve the problems of long growth cycle, difficult to reach plants, small biomass and the like

Active Publication Date: 2021-10-08
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the disadvantages of small biomass and long growth cycle, it is difficult for a single phytoremediation to achieve the desired effect.

Method used

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  • Aspergillus flavus tl-f3 strengthens the method of ryegrass to remediate heavy metal polluted soil
  • Aspergillus flavus tl-f3 strengthens the method of ryegrass to remediate heavy metal polluted soil
  • Aspergillus flavus tl-f3 strengthens the method of ryegrass to remediate heavy metal polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1 Aspergillus flavus TL-F3 strengthens the method of ryegrass repairing heavy metal polluted soil

[0060] 1. Experimental materials

[0061] Tetraploid extra-high annual ryegrass (Dongmu 70) was purchased from Jilin Province Kaiyuan Seed Industry Co., Ltd.

[0062] The strain is Aspergillus flavus TL-F3 with the preservation number CGMCC No.8146.

[0063] The soil was collected near a mining area in Tongling City, Anhui Province. The physical and chemical properties of the soil: pH value is 6.5, total nitrogen is 1.21g / kg, total phosphorus is 0.14g / kg, total potassium is 0.16g / kg, organic matter is 8.74g / kg kg, EC is 250.00mS / cm. The initial concentrations of heavy metals Pb(II), Zn(II), Cu(II) and Cd(II) in soil were 16.17, 171.74, 330.91 and 0.30 mg / kg, respectively.

[0064] Select an appropriate amount of A.flavus TL-F3 inclined plane and plate, add sterile normal saline (0.85% NaCl solution) to fully wash the spores, filter the washing solution with ...

Embodiment 2

[0098] Example 2 Aflavus TL-F3 resistance test to Pb(II) and Cd(II)

[0099] Experimental method: 1mL concentration is 10 7 -10 8 Spores / mL A.flavus TL-F3 spore liquid was added to the liquid medium containing different concentrations of Pb(II) and Cd(II) respectively, and cultured on a shaker at 30°C and 130r / min for 7 days. The concentrations of Pb(II) were 0, 50, 100, 250, 500, 700, 900 and 1000mg / L; the concentrations of Cd(II) were 0, 10, 20, 40, 80 and 100mg / L. Filter the mycelial balls obtained by cultivation, clean them with deionized water, dry them in a 60°C oven to constant weight, and weigh the growth of the bacteria according to the gravimetric method ( Figure 10 and Figure 11 ), the minimum inhibitory concentrations of Pb(II) and Cd(II) to the growth of A.flavus TL-F3 cells were determined to be 1000mg / L and 100mg / L, respectively.

[0100] Liquid medium for bacterial growth: glucose 20g, peptone 10g, NaCl 0.2g, CaCl 2 0.1g, KCl0.1g, K 2 HPO 4 0.5g, NaH...

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Abstract

The invention discloses a method for strengthening Aspergillus flavus TL-F3 to restore heavy metal-contaminated soil with ryegrass. The method comprises combining Aspergillus flavus TL-F3 and ryegrass to repair heavy-metal composite polluted soil. The present invention finds for the first time that Aspergillus flavus TL‑F3 has a growth-promoting effect on plants, and the concentrations of Aspergillus flavus TL‑F3 at Pb(II), Zn(II), Cu(II) and Cd(II) are respectively 50, 200, 300 and 5mg / L still has a good growth-promoting effect. Inoculation of Aspergillus flavus TL‑F3 significantly promoted the growth of ryegrass, increased the enrichment efficiency of ryegrass to Pb(II), Zn(II), Cu(II) and Cd(II), and reduced the concentration of heavy metals in soil. The invention provides a new method for remediation of heavy metal compound polluted soil.

Description

technical field [0001] The invention relates to the technical field of remediation of heavy metal-contaminated soil, in particular to a method for strengthening ryegrass with Aspergillus flavus TL-F3 to remediate heavy metal-contaminated soil. Background technique [0002] In the soil ecosystem, the sources of heavy metals are very extensive, mainly in two ways: natural sources and anthropogenic sources. Among natural factors, natural disasters such as earthquakes, mudslides, and volcanic eruptions have a great impact on the content of heavy metals in soil. Among the human factors, the heavy metal content in soil caused by industry, agriculture, transportation and other sources accounts for a relatively high proportion, and is the main source of soil heavy metal pollution. Heavy metal pollution in the environment is often not caused by a single heavy metal, but accompanied by compound pollution of multiple heavy metals. Due to the characteristics of concealment, complexity...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/34A01P21/00C09K17/14C05F17/20C05G3/80B09C1/10C09K101/00
CPCA01N63/34B09C1/105C05F5/002C09K17/14C09K2101/00C05F17/20C05G3/80C05F11/08Y02W30/40Y02A40/10
Inventor 樊霆刘如潘丹丹陈海燕张祎雯刘小强叶文玲汤婕张伟王振范世锁
Owner ANHUI AGRICULTURAL UNIVERSITY
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