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A kind of biological antibacterial agent of emulsified water-based metalworking fluid and preparation method thereof

A biological antibacterial agent and metal-based technology, which is applied in the field of emulsified water-based metalworking fluid biological antibacterial agent and its preparation, can solve the problems of emulsified water-based metalworking fluids such as easy corruption, and reduce the environment and human body. hazards, prolonging the service life, and high economic benefits

Active Publication Date: 2021-08-13
TIANJIN RES INST FOR ADVANCED EQUIP TSINGHUA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention aims to propose a biological antibacterial agent for emulsified water-based metalworking fluid and its preparation method, to solve the problem of easy corruption of emulsified water-based metalworking fluid, and to provide a The biological antibacterial agent based on metalworking fluid, the microbial antibacterial agent involved can be directly added to the metalworking fluid to prolong the service life of the metalworking fluid on the basis of not affecting the antirust, lubrication and other properties of the metalworking fluid. service life

Method used

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  • A kind of biological antibacterial agent of emulsified water-based metalworking fluid and preparation method thereof
  • A kind of biological antibacterial agent of emulsified water-based metalworking fluid and preparation method thereof
  • A kind of biological antibacterial agent of emulsified water-based metalworking fluid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take the biological antibacterial agent of emulsified water-based metalworking fluid prepared by strain Y1 (Pseudomonas aeruginosa) as an example.

[0032] The mass percent of each component in the biological antibacterial agent is: 2% of microbial cells, 0.05% of component A; 0.7% of component B; 0.6% of component C; and the rest is sterilized deionized water. Wherein, microbial thallus selects Pseudomonas aeruginosa, component A is phenazine, alkaline protease (the mass ratio of the two is 3:2), component B is valine, and component C is ascorbic acid, rhamnolipid ( The mass ratio of the two is 1:2).

[0033] The colony morphology of strain Y1 (Pseudomonas aeruginosa) is as follows figure 1 As shown, the colony is brown and the surface is wet. The preparation method of the biological antibacterial agent containing bacterial strain Y1 comprises the steps:

[0034] (1) Under aseptic operation conditions, pick the slant-preserved strains, line the three zones onto the ...

Embodiment 2

[0039] Take the biological antibacterial agent of emulsified water-based metalworking fluid prepared by strain H3 (Lactobacillus brevis) as an example.

[0040] The mass percent of each component in the biological antibacterial agent is: 3% of microbial cells, 0.04% of component A; 0.6% of component B; 0.8% of component C; and the rest is sterilized deionized water. Among them, the microbial cells are Lactobacillus brevis, component A is lactobacillus peptide, component B is aspartic acid and arginine (the mass ratio of the two is 1:1), and component C is polyvinylpyrrolidone and inositol (The mass ratio of the two is 3:1).

[0041] The preparation method of the biological antibacterial agent containing bacterial strain H3 (lactobacillus brevis) comprises the steps:

[0042] (1) Under aseptic operation conditions, pick the slant to preserve the bacterial strains, draw the three-section line onto the LB plate medium, activate and culture at 36°C for 24 hours, and obtain a sing...

Embodiment 3

[0047] Take the biological antibacterial agent of emulsified water-based metalworking fluid prepared by strain D5 (Proteus) as an example.

[0048] The mass percent of each component in the biological antibacterial agent is: 5% of microbial cells, 0.07% of component A; 0.8% of component B; 0.7% of component C; and the rest is sterilized deionized water. Wherein, the microbial thalline is selected Proteus, component A is Proteus, elastase (the mass ratio of the two is 4:3), and component B is proline, leucine (the mass ratio of the two is 5:3). ), component C is glutaric acid.

[0049] The preparation method of the biological antibacterial agent containing bacterial strain D5 (proteus) comprises the steps:

[0050] (1) Under aseptic operation conditions, pick the slant to preserve the bacterial strains, draw the three-section line onto the LB plate medium, activate and culture at 36°C for 24 hours, and obtain a single colony.

[0051] (2) Pick a single colony from the LB plat...

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Abstract

The invention provides a biological bacteriostatic agent for an emulsified water-based metal working fluid and a preparation method thereof, comprising microbial cells and auxiliary agents; the microbial cells are Bifidobacterium, Lactobacillus brevis, Pseudomonas aeruginosa, Pseudomonas One or more of Bacillus and Clostridium butyricum; the auxiliary agent includes component A, component B and component C, and the component A is lactobacillus, phenazine, proteobactin, alkaline protease , one or more of elastase, the component B is one or more of aspartic acid, arginine, valine, proline, lysine, leucine, so The component C is one or more selected from polyvinylpyrrolidone, inositol, ascorbic acid, rhamnolipid and glutaric acid. The biological bacteriostatic agent of the invention can not only effectively prolong the service period of the metal working fluid, but also reduce the harm of the chemical bactericide to the environment and human body.

Description

technical field [0001] The invention belongs to the field of metal working fluids, and in particular relates to a biological antibacterial agent for emulsified water-based metal working fluids and a preparation method thereof. Background technique [0002] Metalworking fluid refers to the liquid that plays the role of cooling, lubricating, antirust, cleaning, etc. in the process of metal processing, usually composed of most mineral oil, vegetable oil, synthetic oil and other base oils and a small amount of various additives. There is a key problem in the emulsified metalworking fluid, that is, the O / W emulsified working fluid is easy to infect microorganisms and prone to corruption. This is because the base oil and some additives in the metalworking fluid are nutrients for the survival and reproduction of microorganisms. substance. When microorganisms reproduce in large numbers, the pH value of the metalworking fluid will drop, the lubricating performance and anti-rust perf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C10M159/00C10M167/00C10M163/00C10M173/02C12N1/20C10N40/24C10N30/16C12R1/385C12R1/24C12R1/01
CPCC10M159/00C10M163/00C10M167/00C10M173/02C10M2207/022C10M2207/044C10M2207/123C10M2209/12C10M2215/04C10M2215/14C10M2215/221C10M2215/223C10M2217/028C10M2217/044C12N1/20
Inventor 赵朋飞李小磊戴媛静张晨辉雒建斌
Owner TIANJIN RES INST FOR ADVANCED EQUIP TSINGHUA UNIV
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