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Laccase-nanoflower-gamma-Fe2O3 copper alginate gel beads and preparation method and application thereof

A copper alginate, -fe2o3 technology, applied in biochemical equipment and methods, chemical instruments and methods, water/sludge/sewage treatment, etc., can solve the complex synthesis process, chlorophenol compounds are difficult to degrade and consume. Energy and other issues, to achieve the effect of good stability and good operating stability

Active Publication Date: 2020-09-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chlorophenol compounds are difficult to be degraded by microorganisms and are serious pollutants
2.4-Dichlorophenol, 4-p-chlorophenol, and 2-o-chlorophenol have a strong denaturing effect on organisms, are irritating to the skin and mucous membranes, and are corrosive, seriously endangering health
[0007] However, in the synthesis of mesoporous silica microspheres, block copolymers (P123) need to be used as templates to mediate the appearance of mesoporous structures, resulting in higher synthesis costs of mesoporous materials, and high temperature calcination is required after synthesis. Removing templates not only consumes energy but also requires equipment such as muffle furnaces and reactors, which makes the synthesis process relatively complicated
[0008] In addition, using Fe 3 o 4 @SiO 2 -NH 2 or Fe 2 o 3 @MSS-NH 2 When used as a carrier, the amino group on the laccase is covalently linked to the amino group on the surface of the carrier through the aldehyde group on the bifunctional cross-linking reagent. less active than free enzyme

Method used

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  • Laccase-nanoflower-gamma-Fe2O3 copper alginate gel beads and preparation method and application thereof
  • Laccase-nanoflower-gamma-Fe2O3 copper alginate gel beads and preparation method and application thereof
  • Laccase-nanoflower-gamma-Fe2O3 copper alginate gel beads and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A Laccase-Nanoflower-γ-Fe 2 o 3 The preparation method of copper alginate gel beads, comprising:

[0039] (1) Synthesis of laccase-copper phosphate hybrid nanoflowers

[0040] Use phosphate buffer (pH7.0, 0.05M) to prepare 3000mL of laccase solution with a concentration of 0.1mg / mL, and then add 20mL of CuSO 4 Solution (120mM), the above mixture was left at room temperature for 72h, and the precipitate was separated from the solution by centrifugation to obtain laccase-copper phosphate hybrid nanoflowers.

[0041] (2) γ-Fe 2 o 3 Synthesis

[0042] Ferric chloride (200mL, 0.4M) and ferrous chloride (200mL, 0.5M) were added to ammonia solution (200mL, 0.2M), and the precipitate was separated from the solution by centrifugation. In order to obtain an acidic sol, the precipitate was added to Stir in 0.25M perchloric acid aqueous solution for flocculation, so that Fe 3 o 4 The particles are aggregated and precipitated, and then oxidized by ferric nitrate at 90°C, and...

Embodiment 2

[0048] A Laccase-Nanoflower-γ-Fe 2 o 3 The preparation method of copper alginate gel beads is basically the same as the preparation method of Example 1, the difference is: in the laccase-nanoflower-γ-Fe 2 o 3 During the synthesis of copper alginate gel beads, the amount of laccase-copper phosphate hybrid nanoflowers used was 25 mg.

Embodiment 3

[0050] A Laccase-Nanoflower-γ-Fe 2 o 3 The preparation method of copper alginate gel beads is basically the same as the preparation method of Example 1, the difference is: in the laccase-nanoflower-γ-Fe 2 o 3 During the synthesis of copper alginate gel beads, the amount of laccase-copper phosphate hybrid nanoflowers used was 10 mg.

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Abstract

The invention discloses laccase-nanoflower-gamma-Fe2O3 copper alginate gel beads and a preparation method and application thereof. The gel beads are obtained through the steps of stirring sodium alginate, gamma-Fe2O3 ferrofluid colloid solution and laccase-cupric phosphate hybridization nanoflowers in water, and dropwise adding a copper chloride solution at constant speed. The mass ratio of the sodium alginate to the gamma-Fe2O3 ferrofluid colloid solution to the laccase-cupric phosphate hybridization nanoflowers is 3000: (30000-70000):(5-50); in the gamma-Fe2O3 ferrofluid colloid solution, the particle diameter of gamma-Fe2O3 particles is 10nm, and the diameters of the laccase-cupric phosphate hybridization nanoflowers are 15-20[mu]m. The laccase-nanoflower-gamma-Fe2O3 copper alginate gelbeads show higher catalyzing efficiency than free enzymes in reaction of catalyzing degradation of chlorophenol, and oxidizing polycyclic aromatic hydrocarbon and phenolic compounds, and show favorable reusability and storage stability.

Description

technical field [0001] The invention relates to a copper alginate gel bead, in particular to a laccase-nano flower-γ-Fe 2 o 3 Copper alginate gel beads, preparation method and application thereof. Background technique [0002] Chlorophenols are toxic. As the number of chloride ions increases, their toxicity increases, and they are more difficult to be oxidized. Chlorophenols are difficult to be degraded by microorganisms and are serious pollutants. 2. 4-dichlorophenol, 4-p-chlorophenol and 2-o-chlorophenol have a strong denaturing effect on organisms, are irritating to the skin and mucous membranes, and are corrosive, seriously endangering health. [0003] Polycyclic aromatic hydrocarbons are a class of hydrocarbons containing two or more benzene rings. Studies have shown that various polycyclic aromatic hydrocarbons are carcinogenic, mutagenic and reproductively toxic. PAHs are mainly produced by incomplete combustion of organic materials, such as factory exhaust gas, v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/04C02F3/34C02F101/34C02F101/36C02F101/32
CPCC12N11/14C12N11/10C12N11/04C12N9/0061C02F3/342C12Y110/03002C02F2101/345C02F2101/36C02F2101/327
Inventor 吴卓夫王君吕海艳张斯童于潇潇
Owner JILIN AGRICULTURAL UNIV
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