Raspberry quality marker and preparation method thereof

A technology of raspberries and markers, applied in the field of medicine, can solve the problems of few reports on quality control, limited one-sided quality control, poor specificity of indicators, etc.

Pending Publication Date: 2020-09-18
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The 2015 edition of the Chinese Pharmacopoeia controls the quality of raspberries by quantifying the contents of ellagic acid and kaempferol-3-O-rutinoside, but quality control based on pharmacological activity is rarely reported
The traditional quality c

Method used

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  • Raspberry quality marker and preparation method thereof
  • Raspberry quality marker and preparation method thereof
  • Raspberry quality marker and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The chemical composition, main chemical components and some new compounds of raspberry were obtained by using molecular network research method.

[0048] Weigh an appropriate amount of raspberry powder, add 10 times the mass volume of 70% ethanol solution, condense and reflux for 1 hour, take the decoction, filter under reduced pressure, concentrate and dry to obtain a sample of raspberry alcohol extract, weigh 200.0 mg of this sample, dissolve In 1mL of 30% ethanol solution, sonicate and make it fully dissolved evenly, pass this raspberry alcohol solution through SPE small column, elute with 30%, 50%, 70% and 90% ethanol solution respectively, place in 4 centrifuges In the tube, centrifuge at 20000g for 20min and then enter into UPLC-ESI-QTOF-MS system for analysis, chromatographic conditions: chromatographic column is XDB-C18 column (2.1ⅹ100mm, 1.8μM, Agilent), mobile phase is 0.1% formic acid water (A)-acetonitrile ( B), gradient elution: 0-12min, 2%-98%B, 12-14min, ...

Embodiment 2

[0050] Raspberry serum medicinal chemistry research to screen raspberry blood constituents.

[0051] Prepare raspberry ethanol extract, weigh 200.0g raspberry powder, pass through a 40-mesh sieve, add 2000mL 70% ethanol, condense and reflux for extraction for 1h, filter with suction, add 2000mL70% ethanol for raspberry dregs to reflux for 1h, repeat the extraction for 3 Once, merge the decoction, draw 200 μ L ethanol extract, centrifuge 20min under 20000g, draw the supernatant solution as analysis sample, analyze and obtain the BPC chromatogram of raspberry ethanol extract under UPLC-ESI-QTOF-MS ( image 3 , A). Other decoctions were concentrated to dryness by rotary evaporation and set aside. Before the experiment, it is prepared and used now. Weigh an appropriate amount of raspberry ethanol extract dregs, add Wahaha, sonicate until it is completely uniform, and prepare a 1g / mL liquid medicine.

[0052] Rat blank plasma and drug-containing plasma of raspberry ethanol extrac...

Embodiment 3

[0057] The network pharmacology research of raspberry has obtained the target of raspberry in the treatment of renal injury.

[0058]Selected 6 blood components whose structures have been determined as the active ingredients of raspberry to protect acute kidney injury, they are rutin, rutin, silver linden, kaempferol-3-O-rutinoside, ellagic acid and Raspberry glycoside F5. Use ChemBiodraw Ultra 13.0 software to export the chemical structure of each blood component as a .sdf format file, log in to the PharmMapper database and upload the *.sdf format files of the 6 active component structures, set the value of "Maximum GeneratedConformations" to 300, "Select Targets Set" is set to Human Protein Targets Only, "Number of Reserved Matched Targets (Max 1,000)" is set to 300, and the targets of 6 blood-entering components are obtained respectively, and the top 200 targets are screened as candidate targets point. Since the gene name of the target derived from the PharmMapper databas...

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Abstract

The invention discloses a raspberry quality marker and a preparation method thereof. The method is to finally determine a chemical component group with the kidney-protecting efficacy in raspberry by adopting the pharmacological research of the serum pharmaceutical chemistry and the in vivo and in vitro kidney-protecting activity of traditional Chinese medicines. It is found that acute kidney injury induced by cisplatin can be remarkably protected by in vivo administration of the raspberry alcohol extract, and finally, a chemical component group capable of representing the safety and effectiveness of clinical medication of the raspberry is determined by screening the kidney-protecting activity of in vitro chemical components, and the chemical component group can be used as a quality markerof the raspberry, so that the quality control of the raspberry based on the pharmacodynamic components is realized. The invention solves the problems that the medicament curative effect is not clear due to the uneven quality of the raspberry medicinal material and the quality evaluation of the raspberry is not related to the medicament effect of the raspberry.

Description

Technical field: [0001] The invention belongs to the field of medicine and relates to the discovery of quality markers of traditional Chinese medicines, in particular to a raspberry quality marker and a preparation method thereof. Background technique: [0002] Raspberry is the dried fruit of the woody plant Rubuschingii Hu of the Rosaceae Rubus, and it is a health product for both medicine and food. The ancient Chinese book "Shen Nong's Materia Medica" shows that raspberries have the functions of consolidating essence and reducing urine, helping yang and improving eyesight and tonifying kidney. The 2015 edition of the Chinese Pharmacopoeia controls the quality of raspberries by quantifying the contents of ellagic acid and kaempferol-3-O-rutinoside, but quality control based on pharmacological activity is rarely reported. The traditional quality control of traditional Chinese medicine only evaluates its quality through simple fingerprints and quantitative determination of s...

Claims

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Application Information

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IPC IPC(8): G16C20/20G16H70/40
CPCG16C20/20G16H70/40
Inventor 李飞周紫梦王义坤
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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