Application of theanine in preparation of product for relieving alcoholic liver injury
A technology of alcoholic liver injury and theanine, which is applied in the fields of application, function of food ingredients, medical preparations containing active ingredients, etc., can solve problems such as unclear molecular mechanism, achieve the degree of relief of liver injury and relieve acute liver disease. Injury effect, effect of suppressing inflammatory response
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Embodiment 1
[0032] In the present invention, mice are subjected to one-time heavy drinking to make a mouse liver injury model, and the liver injury degree of the mice in the theanine group and the control group given the same dose of sterile water are compared, and the comparison of mouse liver case slices shows that , theanine significantly alleviated the degree of liver damage caused by one-time heavy drinking in mice, significantly reduced the levels of AST and ALT in the serum of mice after heavy drinking, and could increase the levels of superoxide dismutase SOD and gluten in the liver of mice Levels of glutathione peroxidase and decreased levels of malondialdehyde in the liver of hepatic-injured mice.
[0033] At the same time, the inventors studied the molecular mechanism of theanine in alleviating acute alcoholic liver injury in mice, and the results showed that theanine inhibited NF-κB-mediated inflammation by regulating the TNF-α / NF-κB signaling pathway Reaction, inhibit the exp...
Embodiment 2
[0044] (1) The alleviating effect of theanine on alcoholic liver injury in mice
[0045] Liver tissues of mice in each group were stained with hematoxylin and eosin, respectively. Such as figure 1 As shown, compared with the liver tissue of the normal control group (CG), the apoptosis of liver cells in the alcohol-treated model group (MG) was obvious, and the liver tissue was congested, swollen, vacuolated, and granule degenerated. Compared with the model group, the histology and structural morphology of the liver tissue sections of the L-theanine treatment group were improved, especially the liver tissue of the high-dose treatment group was close to the normal group.
[0046] (2) Effect of L-theanine on enzyme activity in mouse serum
[0047] The activity of ALT and AST and the ratio of the two are liver damage, an important marker index of liver inflammation. The inventor detects the activity of ALT and AST in mouse serum, and the results show that ( figure 2 ), compared...
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