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Preparation method of acellular amniotic uterine cavity stent loaded with estradiol microspheres

An estradiol-loaded and decellularized technology, applied in the field of biomedical equipment preparation, can solve the problems of poor treatment effect and high recurrence rate of intrauterine adhesions

Active Publication Date: 2022-03-18
ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of acellular amniotic uterine cavity stent loaded with estradiol microspheres, which is a kind of estradiol-loaded poly The preparation method of the acellular amniotic cavity stent made of lactic acid-glycolic acid copolymer (PLGA) microspheres, the stent can restore the shape of the uterine cavity, effectively repair the endometrium, reduce adverse drug reactions, and realize precise drug delivery in the uterine cavity

Method used

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  • Preparation method of acellular amniotic uterine cavity stent loaded with estradiol microspheres
  • Preparation method of acellular amniotic uterine cavity stent loaded with estradiol microspheres
  • Preparation method of acellular amniotic uterine cavity stent loaded with estradiol microspheres

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Effect test

Embodiment 1

[0028] A. Preparation of acellular amnion matrix:

[0029] (1) Take amniotic membrane tissue: take the placenta negative for hepatitis B, hepatitis C, AIDS, and syphilis under sterile conditions, bluntly separate the amniotic membrane about 10cm×10cm, remove the chorionic membrane tissue, and use 50mg / ml penicillin, 50mg / ml streptomycin Stored after washing with phosphate buffered saline (pH 7.2-7.4).

[0030] (2) Preparation of acellular amniotic matrix: the amniotic membrane was washed repeatedly with normal saline, soaked in the volume fraction polyethylene glycol octylphenyl ether (TritonX-100) solution, placed in a constant temperature incubator at 37°C and shaken overnight, and taken out Then rinse with normal saline, add 25g / L trypsin and 0.2g / L ethylenediaminetetraacetic acid (EDTA), fully shake, and incubate at 37°C for 4h. After taking it out, rinse it repeatedly with normal saline, then place the amniotic membrane tissue in 8.8% sodium chloride solution, incubate a...

Embodiment 2

[0050] A. Preparation of acellular amnion matrix:

[0051] (1) Take amniotic membrane tissue: take the placenta negative for hepatitis B, hepatitis C, AIDS, and syphilis under sterile conditions, bluntly separate the amniotic membrane about 10cm×10cm, remove the chorionic membrane tissue, and use 50mg / ml penicillin, 50mg / ml streptomycin Stored after washing with phosphate buffered saline (pH 7.2-7.4).

[0052] (2) Preparation of acellular amnion matrix: the amnion was rinsed repeatedly with normal saline, soaked in 0.1% TritonX-100 solution by volume fraction, placed in a constant temperature incubator at 37°C and shaken overnight, rinsed with normal saline after taking it out, and added 25g / L trypsin and 0.2g / L EDTA, shake well, and incubate at 37°C for 4h. After taking it out, rinse it repeatedly with physiological saline, then place the amnion tissue in 8.8% sodium chloride solution, incubate at room temperature for 4 hours, take it out, wash it repeatedly with 0.01M phos...

Embodiment 3

[0075] A. Preparation of acellular amnion matrix:

[0076] (1) Take amniotic membrane tissue: take the placenta negative for hepatitis B, hepatitis C, AIDS, and syphilis under sterile conditions, bluntly separate the amniotic membrane about 10cm×10cm, remove the chorionic membrane tissue, and use 50mg / ml penicillin, 50mg / ml streptomycin Stored after washing with phosphate buffered saline (pH 7.2-7.4).

[0077] (2) Preparation of acellular amnion matrix: the amnion was rinsed repeatedly with normal saline, soaked in 0.1% TritonX-100 solution by volume fraction, placed in a constant temperature incubator at 37°C and shaken overnight, rinsed with normal saline after taking it out, and added 25g / L trypsin and 0.2g / L EDTA, shake well, and incubate at 37°C for 4h. After taking it out, rinse it repeatedly with physiological saline, then place the amnion tissue in 8.8% sodium chloride solution, incubate at room temperature for 4 hours, take it out, wash it repeatedly with 0.01M phos...

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Abstract

The invention provides a method for preparing an acellular amniotic uterine cavity stent loaded with estradiol microspheres. Human amniotic extracellular matrix and PLGA estradiol microspheres are combined to construct a bioactive uterine cavity drug slow-release stent. By adjusting the molecular weight of the PLGA polymer and the matrix concentration of the amniotic membrane stent, the estradiol can be completely released within 21 days, achieving synchronization with the estrogen level fluctuations in the female menstrual cycle. The acellular amniotic uterine cavity stent loaded with estradiol microspheres provided by the present invention can be used to prevent adhesion recurrence after hysteroscopic adhesion separation, and to prevent intrauterine adhesion and promote endometrial repair after uterine cavity operation. The uterine cavity stent provided by the invention can not only effectively provide uterine cavity barrier support, but also increase the concentration of estradiol in the uterine cavity, and effectively promote the regeneration of endometrium. The uterine cavity stent has a simple preparation process and is easy to scale up for production, and is expected to become a new pharmaceutical preparation for the prevention and treatment of uterine cavity adhesions.

Description

technical field [0001] The invention belongs to the field of preparation of biomedical equipment, and relates to a method for preparing an acellular amniotic uterine cavity support loaded with estradiol microspheres, which can be used in repairing endometrium and preventing intrauterine adhesions. Background technique [0002] Intrauterine adhesions (IUA), also known as Asherman syndrome, refers to the injury of the basal layer of the endometrium due to trauma or infection, which causes all or part of the uterine cavity wall and (or) cervical canal to be atresiad, and the uterus loses its original structure. A disease with a physiological function. At present, the incidence of IUA in my country remains high, and with the increase of uterine cavity surgery, it shows a trend of increasing year by year. The risk factors for the incidence of IUA include: reproductive system infection, low estrogen environment in the body, history of uterine cavity operations such as abortion cu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/00A61L31/14A61L31/16
CPCA61L31/005A61L31/16A61L31/146A61L31/148A61L31/14A61L2300/222A61L2300/43A61L2300/622A61L2430/40
Inventor 陈玥郑彩虹费伟东叶轶青赵梦丹赵云春张骁吴凡
Owner ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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