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Agave cpSSR marker primers and application thereof

A technology of agave hemp and marker primer, which is applied in the biological field to achieve the effects of increasing original data, improving development and screening efficiency, and enriching types

Active Publication Date: 2020-09-25
GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no information about the development of cpSSR markers of Agave hemp and its application in the analysis of the genetic relationship of Agave hemp

Method used

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  • Agave cpSSR marker primers and application thereof
  • Agave cpSSR marker primers and application thereof
  • Agave cpSSR marker primers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0078] 1.1 Isolation of Agave hemp total DNA

[0079] The high-quality total DNA of agave hemp cultivar H.11648 was extracted by CTAB method, and the absorbance at 230nm, 260nm and 280nm was measured with a NanoDrop2000 micro-ultraviolet spectrophotometer to estimate the purity of the total DNA extracted, and then gelled with 1.0% agarose Gel electrophoresis was used to detect the quality of total DNA. see results figure 1 .

[0080] 1.2 Completion of the chloroplast genome of Agave hemp H.11648

[0081] The total DNA of Agave hemp cultivar H.11648 was used to construct PE library. DNA Fragmentation DNA was fragmented using a Covaris instrument.

[0082] The fragmented DNA fragments were concentrated and recovered using Agencourt AMPure XP-Nucleic Acid Purification Magnetic Beads. Construction of the Illumina P150 library.

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Abstract

The invention discloses development and application of agave cpSSR marker primers. The cpSSR marker sequences corresponding to SEQ ID NO.21 to SEQ ID NO.40 of the primer pair are developed based on sequencing of a chloroplast genome of an agave cultivar H.11648, the developed cpSSR marker sequences and a homologous sequence of a published chloroplast genome species of agave are subjected to multiple comparison analysis, polymorphic cpSSR site screening and cpSSR marker primer design are carried out, and polymorphic primers are applied to agave resource evaluation through sequencing and comparison of PCR products. According to the patent, the problem of low development efficiency of a traditional cpSSR polymorphism marker is solved, and the blank of agave cpSSR marker information at presentis filled; and the developed cpSSR polymorphic marker primers lay a foundation for research of systematic classification, genetic diversity evaluation, germplasm identification and the like of the agave.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the development and application of a polymorphic cpSSR marker primer of Agave hemp. Background technique [0002] Agave hemp, also known as sisal hemp, is a general term for monocotyledonous plants of the genus Agave. According to current knowledge, there are 257 species of Agave plants in the world, and about 50 species have important economic value. The leaves of certain plant species of Agave are rich in fiber, and the fiber has the characteristics of white color, tough texture, high elasticity, strong pulling force, abrasion resistance, acid and alkali resistance, corrosion resistance, and not easy to slip. Due to the above-mentioned characteristics, agave hemp fiber becomes the largest and most widely used hard fiber in the world today. [0003] In 1963, my country introduced the high-yielding fine variety H.11648 from East Africa. After being planted properly, it has been pop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6809C12Q1/6858C12N15/11G16B20/30G16B25/20G16B30/10G16B30/20
CPCC12Q1/6895C12Q1/6809C12Q1/6858G16B20/30G16B25/20G16B30/10G16B30/20C12Q2600/156C12Q2535/122C12Q2531/113C12Q2535/101
Inventor 金刚陈涛覃旭吴密黄显雅覃剑峰彭欣怡黄锦媛
Owner GUANGXI SUBTROPICAL CROPS RES INST GUANGXI SUBTROPICAL AGRI PROD PROCESSING RES INST
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