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Cell-containing biological gel composition and preparation method and application thereof

A biogel and composition technology, applied in the field of biomedical materials, can solve the problems of limited cell compatibility, short degradation time, and inability to well regulate cell functions, and achieve good temperature sensitivity and maintain activity. Effect

Inactive Publication Date: 2020-09-29
北京奥泰康医药技术开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned materials have problems such as too short degradation time, limited cell compatibility, and inability to regulate cell functions well.
However, there are no related reports on the use of omentum acellular matrix biogel as a carrier combined with adipocytes to establish a biogel injection transplantation system and apply it to improve the residence and survival of adipocytes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0074] (3) Preparation of Omentum Acellular Matrix Digestion Solution

[0075] Dissolve trypsin, collagenase and dispaseII in PBS solution at a mass ratio of 2:2:1-2, wherein the concentrations of trypsin, collagenase and dispaseII in PBS solution are all 0.05-0.25%, after complete dissolution , adjust the pH to 7.2-7.4 to obtain omentum decellularized matrix digestion solution.

[0076] (4) Omentum decellularization treatment

[0077] 1) Take fresh omentum tissue, wash it in phosphate buffered saline, and soak the cleaned tissue in the degreasing solution for treatment. The soaking time in the degreasing solution is 18-30 hours, and the oscillation frequency is 150-250r / min ;

[0078] 2) Wash the omentum tissue from which fat has been removed in phosphate buffered saline, and oscillate and soak in the decellularized solution I for treatment. The immersion time is 4-12 hours, and the oscillating frequency is 100-200r / min;

[0079] 3) Wash the omentum tissue treated with the...

Embodiment 1

[0100] Example 1 Decellularization of omentum derived from miniature pigs

[0101] Fresh minipig peritoneal omentum tissues (purchased from slaughterhouses) were taken and washed 3 times in PBS. The cleaned tissues were soaked in 2L of degreasing solution for 24 hours by shaking, and the shaking frequency was 200r / min. Subsequently, the fat-removed omentum tissue was washed three times in PBS, and soaked in 1L of decellularized solution I for 8 hours with shaking at a frequency of 150 r / min. Subsequently, the omentum tissue treated with decellularization solution I was washed 3 times in PBS, and soaked in 1L decellularization solution II for 8 hours with shaking at a frequency of 150r / min. Subsequently, the omentum tissue treated with decellularization solution II was washed 3 times in PBS, and soaked in 1L decellularization solution III for 8 hours with shaking at a frequency of 150r / min. Finally, the omentum tissue treated with decellularization solution III was washed wit...

Embodiment 2

[0102] The preparation of embodiment 2 omentum acellular matrix biogel

[0103] Add 10 g of the washed and shredded omentum acellular matrix to the digestion bottle containing the above digestion solution, then add 100 ml of the digestion solution, cover it and put it on a magnetic stirrer to stir at a speed of 100 rpm. The magnetic stirrer and The digestion flasks were placed together in a 37°C incubator for continuous digestion for 30-45min, and then 2mL of serum was added to terminate the digestion reaction, wherein the volume ratio of serum to digestion solution was 0.1-0.2:10. The digestive juice was collected to obtain the liquid omentum acellular matrix biogel. Adjust the omentum acellular matrix concentration to 20mg / mL, and form a gel-like omental acellular matrix in 5-10 minutes at 37°C

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Abstract

The invention relates to the field of biomedical materials, in particular to a cell-containing biological gel composition and a preparation method and application thereof. Compared with the existing normal saline-carrying fat cell transplantation, the biological gel composition compounded with fat cells established by the method of the invention contains a variety of cytokines and has good temperature sensitivity, and after the biological gel composition carrying the fat cells is transplanted into the body, the biological gel composition can be changed from a liquid state to a gel state withina few minutes, so that the fat cells stay at an injection site without losing, and at the same time the activity of the fat cells is maintained. A new injection transplantation system is provided forthe basic research and clinical application of the plastic repair surgery.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a biogel composition containing cells, a preparation method and application thereof. Background technique [0002] Clinically, the repair of soft tissue defects caused by congenital malformations, trauma, tumor resection, and cosmetic needs is the most common problem faced by plastic and reconstructive surgery. In order to solve the above problems, collagen injection, dermis transplantation, artificial synthetic materials and free adipose tissue transplantation have been widely used in clinical practice. At present, there are many soft tissue defect filling materials commonly used clinically, including collagen, hyaluronic acid, polyacrylamide hydrogel, polylactic acid, etc. , which limits its clinical application. [0003] Autologous fat transplantation refers to a plastic method that obtains granular fat tissue through liposuction and injects it into local soft tissue to i...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/38A61L27/50A61L27/52
CPCA61L27/3604A61L27/3687A61L27/3691A61L27/38A61L27/50A61L27/52A61L2400/06A61L2430/34A61L2430/40
Inventor 尹玉静饶义伟
Owner 北京奥泰康医药技术开发有限公司
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