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A preparation method of signal-enhanced immunosensor based on lanthanide metal self-luminescence au@gd-mofs

An immunosensor and signal enhancement technology, applied in the field of preparation of enhanced electrochemiluminescence immunosensors

Active Publication Date: 2022-08-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of constructing a signal-enhanced ECL immunosensor based on the self-luminescent nanomaterial Au@Gd-MOFs to detect PCT has not been reported.

Method used

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  • A preparation method of signal-enhanced immunosensor based on lanthanide metal self-luminescence au@gd-mofs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Nano -nano PT@ni (OH) gathered by ultra -thin nano tablets 2 , Self-Optical Nano-Pore Material AU@GD-MOFS Label PCT Puppet coupling original incubation original incubation solution

[0033] (1) Nanoman PT@ni (OH) concentrated by ultra -thin nano -slices 2 Preparation

[0034] Add 1 mg hexal nickel nitrate nickel, 1 G heyhnamide tetramine (HMT) to 35 mL ultra -pure water, stir with magnetic force for 5 minutes, get a mixture, take 5 cm*1.5 cm*0.1 cm nickel 1-propylene: pyruis: water (1: 1: 1) ultrasonic 5min, go out of the oil layer on the surface of nickel nickel, and continue to immerse in 0.1 mmol L -1 In the hydrochloric acid solution, ultrasonic 5 min remo the surface oxide, ultra -pure water, ethanol was washed in sequence, and nitrogen blows dry at low temperature. It is weighed. Together with the above mixture to 50 ml of polytetrafluoroethylene lining high -pressure reactor, 80 ℃ The reaction 8 h, get the target product, freeze and dry, that is, the nano PT@ni (OH) ...

Embodiment 2

[0042] Nano -nano PT@ni (OH) gathered by ultra -thin nano tablets 2 , Self-Optical Nano-Pore Material AU@GD-MOFS Label PCT Puppet coupling original incubation original incubation solution

[0043] (1) Nanoman PT@ni (OH) concentrated by ultra -thin nano -slices 2 Preparation

[0044] Add 1.5 mg hexal nickel nitrate nickel, 1.5 G heylumineticoramine (HMT) to 35 mL ultra -pure water, mix the magnetic force for 10 min, get a mixture, take 5 cm*1.5 cm*0.1 cm nickel, Immersed 1-propyol: Pitthol: water (1: 1: 1) ultrasonic 10 min, go out of the oil layer on the surface of nickel nickel, and continue to immerse in 0.25 mmol L -1 In the hydrochloric acid solution, the ultrasonic 10 min removes the surface oxide, washed in ultra -pure water and ethanol, and the nitrogen blows dry at low temperature. It is weighed. Together with the above mixture to 50ml polytefluoroethylene, 10 h, get the target product, freeze and dry, that is, nano -nano PT@ni (oh) gathered from ultra -thin nano -slices 2...

Embodiment 3

[0052] Nano -nano PT@ni (OH) gathered by ultra -thin nano tablets 2 , Self-Optical Nano-Pore Material AU@GD-MOFS Label PCT Puppet coupling original incubation original incubation solution

[0053] (1) Nanoman PT@ni (OH) concentrated by ultra -thin nano -slices 2 Preparation

[0054] Add 2 mg hexal nickel nitrate nickel and 2 G heyhnamine tetramine (HMT) to 35 mL ultra -pure water, stir the magnetic force for 15min, get a mixture, take 5 cm*1.5 cm*0.1 cm nickel 1-propylene: pyruis: water (1: 1: 1) 15min of ultrasonic, go out of the oil layer on the surface of nickel nickel, and continue to immerse in 0.5 mmol L -1 In the hydrochloric acid solution, the ultrasonic 15 min removes the surface oxide, washed in ultra -pure water and ethanol, and the nitrogen blows dry at low temperature. It is weighed. Together with the above mixture to 50 ml of polytrafluoroethylene lining high -pressure reactor, 120 ℃ The reaction is 12 h, the target product is obtained, and the nano PT@ni (OH) is mad...

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Abstract

The invention relates to a preparation method of a signal-enhanced electrochemiluminescence immunosensor based on a novel self-luminous nanoporous material Au@Gd-MOFs, belonging to the field of electrochemiluminescence sensors. As an electrochemiluminescence signal source, nanoflowers Pt@Ni(OH) aggregated by ultrathin nanosheets 2 Compared with the traditional two-dimensional sheet material, it has a larger specific surface area and exposes more active sites to increase the immobilization capacity of the antibody. According to the difference in the electrochemiluminescence signal intensity caused by different concentrations of antigen, the detection of PCT in human serum is realized.

Description

Technical field [0001] The present invention involves a preparation method of enhanced electrochemical chemical emitting immune sensor based on the@GD-MOFS of the 镧 镧@@本. Specifically involved the nanoma material AU@GD-MOFS and the base ultra-thin nano piece PT@ni (oh) 2 The application of preparation and its application in electrical chemical emitting sensors. Among them, ultra -thin nano -pt@ni (oh) 2 The flower-shaped shape of the gathering is rich in more active sites than the simple two-dimensional base film-like material. The surface area and high pore rate reaches the purpose of enlarging the sensor light signal output and widening the linear range of detection. The invention is the field of electrochemical lighting testing technology. Background technique [0002] ProcalCitonin (PCT) is a large molecular protein in human plasma. The concentration in healthy individuals is very low (≤ 0.1 ng ml), but under the induction of cytokines such as internal toxins, 2 h ~ 3 H start...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327G01N27/30G01N21/76G01N33/74G01N33/58
CPCG01N27/48G01N27/3278G01N27/308G01N21/76G01N33/74G01N33/587G01N2333/585G01N2800/7095
Inventor 赵冠辉杜宇王欢任祥曹伟魏琴范大伟鞠熀先
Owner UNIV OF JINAN