Formula of biological microbial inoculum suitable for preventing and treating tomato root-knot nematode diseases

A tomato root-knot nematode and biological agent technology, applied in the field of microorganisms, can solve the problems of live spores that are harmful to human health, unstable control effects, and easy inactivation of live bacteria, so as to achieve good utilization prospects and alleviate tomato root knots. Nematode disease, the effect of easy application

Pending Publication Date: 2020-10-02
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of biological bacterial agent of control tomato root-knot nematode disease, can replace existing biological control agent in actual tomato production process; Bacteria biological preparations live ba

Method used

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  • Formula of biological microbial inoculum suitable for preventing and treating tomato root-knot nematode diseases

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Embodiment 1

[0017] The preparation of embodiment 1 Paecilomyces lilacinus metabolite biological agent

specific Embodiment approach 1

[0018] Specific embodiment one: the preparation of different Paecilomyces lilacinus liquid medium

[0019] Take 10g of Jerusalem artichoke stalk debris, add 100ml of distilled water, incubate at 28°C and 200r / min for 24 hours, then filter with three layers of filter paper, and then filter with a 0.45mm microporous membrane, and the obtained filtrate is the extract of Jerusalem artichoke stalks , placed in a -80°C refrigerator for future use.

[0020] Paecilomyces lilacinus 84604 was inoculated in PDA medium to allow the strain to grow fully. After 7 days of activation, the concentration was 1x10 7 / ml of spore suspension. Adding 0%, 0.1%, 0.3%, and 0.5% concentration of Jerusalem artichoke stalk extract in four medium formulations of I, II, III, and IV respectively to cultivate Paecilomyces lilacinus, totally 16 formulations (such as figure 1 ).

specific Embodiment approach 2

[0021] Specific embodiment two: preparation of different Paecilomyces lilacinus metabolites biological agents

[0022] Take 1% of the total amount of the liquid culture medium and inoculate the spore suspension into each culture medium, cultivate at 28°C and 180r / min for 7 days to obtain a fermented liquid, and coarsely filter each fermented liquid through three layers of filter paper to obtain the filtrate Centrifuge at 8000 rpm for 10 min, and finally filter through a 0.22 μm microporous membrane. The filtrate obtained after filtration is the metabolite of Paecilomyces lilacinus (mixed component). Dilute the metabolites of Paecilomyces lilacinus obtained by doubling to make a biological agent of metabolites of Paecilomyces lilacinus, and store them in a refrigerator at 4°C for future use (it can also be made into freeze-dried powder for storage).

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Abstract

The invention relates to a preparation method and an application of a biological microbial inoculum suitable for preventing and treating tomato root-knot nematode diseases, and belongs to the field ofmicroorganisms. The preparation method of the biological microbial inoculum comprises the following steps: preparing a liquid culture medium which comprises the following components in percentage byconcentration: 1.0 percent of sodium nitrate, 0.50 percent of peptone, 0.005 percent of magnesium sulfate heptahydrate, 0.50 percent of ammonium sulfate, 0.05 percent of potassium dihydrogen phosphate, 4.0 percent of glucose and 0.3 percent of jerusalem artichoke straw extract; performing inoculating 1*10 <7>/ml of spore suspension on the prepared liquid culture medium, performing culturing for 7days under the conditions of 28 DEG C and 180r/min, performing filtering, and diluting by one time to obtain the paecilomyces lilacinus metabolite biological microbial inoculum. The biological microbial inoculum prepared by adopting a technology of compounding the organic matter with the nematode killing effect and the culture medium can effectively reduce the occurrence of tomato root knot nematode diseases and promote the growth of tomatoes, and has the advantages of simplicity and convenience in application, batch production, no parasitic influence, high safety and the like; and good application prospects and development potentials are realized in the aspect of relieving tomato root knot nematode diseases.

Description

technical field [0001] The invention relates to the preparation of a biological bacterial agent by using an extract of Jerusalem artichoke stalks and a preparation method thereof, and belongs to the field of microorganisms, in particular to the preparation of a biological bacterial agent of a metabolite of Paecilomyces lilacinus suitable for preventing and curing tomato root-knot nematode diseases. Background technique [0002] Tomato is an annual or perennial herbaceous plant belonging to the family Solanaceae. As one of the main fruit vegetables in the world, tomato has high nutritional value and has been widely planted all over the world. Due to its large demand and limited planting area, continuous cropping has become a common phenomenon. However, long-term continuous cropping cultivation will affect tomato by continuous cropping obstacles, resulting in imbalance of soil microbial community structure, changes in soil physical and chemical properties, and seriously affec...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P5/00A01P21/00C12R1/79
CPCA01N63/30C12N1/14
Inventor 潘凯任晶李大龙吴凤芝
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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