Method for preparing microbiological micro-ecological inoculum by using corn steep liquor as raw materials through symbiosis fermentation technique

Abstract

The invention discloses a method for preparing microbiological micro-ecological inoculum by using corn steep liquor as raw materials through a symbiosis fermentation technique, and relates to the fields of biological technology and microecology, and mainly solves the problems to be difficult to treat, that the corn steep liquor is high in acidity, toxins easily exceed the standard, and the added value is low. The method can promote resource reutilization of corn steep liquor waste. The microbiological micro-ecological inoculum is liquid microbiological micro-ecological inoculum. The preparation steps mainly adopt the components of 10-30 parts of corn steep liquor, 1-4 parts of corn flour, 0.1-0.5 part of magnesium sulphate, 1-2 parts of potassium hydroxide and the balance water. Under thecondition that inoculum concentration of bacillus subtilis is 1-5%, the inoculum concentration of bacillus licheniformis is 2-8%, the inoculum concentration of aspergillus niger is 2-8%, the fermentation temperature is 28-35 DEG C, the rotating speed is 120-180rpm, and the liquid loading amount is 1/2 to 1/5, fermentation is performed for 2-3 days. A potassium hydroxide neutralizing scheme is adopted, and the symbiosis fermentation technique is utilized, so that a high activity multi-bacteria microbiological system is realized, and finally, high cell amount and effects of removing phosphorus and potassium can be obtained. According to the technique, the added value of core processed by-products can be increased, besides, the obtained microbiological micro-ecological inoculum product can beused for preparing a biological organic fertilizer, and crop growth is promoted.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the field of micro-ecology, and in particular relates to a method for comprehensively utilizing corn starch processing by-products—corn steep liquor symbiotic fermentation to prepare micro-ecological bacterial agents. Background technique [0002] my country is the second largest corn producing country next to the United States. Except for direct consumption, most of the corn produced every year is used as raw materials for the food industry. At present, the deep processing of corn mainly starts from the processing of corn starch, which produces a large amount of by-products of corn starch processing. my country processes about 50 million tons of corn raw materials every year, of which the waste generated from the production of corn starch-corn steep liquor exceeds 2 million tons. [0003] At present, the processing of corn steep liquor has become the bottleneck of the enterprise. A part o...

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Problems solved by technology

[0006] In view of this, the present invention aims at the problems that corn steep liquor is difficult to use and easily causes environmental pollution and waste of resources, and provides a method of using corn steep liquor as raw material to prepare micro-ecological bacterial agents using symbiotic fermentation technology, increasing the added value of corn deep processing industry, improving Soil quality, promotes plant growth