A kind of hybrid material modified by glutathione and its preparation and application
A hybrid material, glutathione technology, applied in the preparation methods of peptides, peptides, organic chemistry, etc., can solve the problems of long cycle and cumbersome preparation process, and achieve the effect of low cost of raw materials
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Embodiment 1
[0019] Example 1 The glutathione-functionalized hybrid material is used for the separation and enrichment of glycopeptides.
[0020] Preparation of glutathione-functionalized hybrid materials:
[0021] 1) Preparation of vinyl-functionalized hybrid materials: First, weigh 5 mg of dodecylamine and 20 mg of CTAB into a centrifuge tube, add 175 μL of ethanol and 125 μL of water, ultrasonically dissolve to form a homogeneous solution, and then add 100 mg of Tetraethoxysilane and 100mg 7-octenyltrimethoxysilane were placed in a water bath at 40°C for 24 hours; the hybrid material was washed three times with ethanol / water solution (1 / 1, v / v) , Vacuum drying at 60°C for 12h to obtain a vinyl-functionalized hybrid material.
[0022] 2) Preparation of glutathione-modified hybrid material: First, 20 mg of glutathione and 2 mg of photoinitiator DMPA were dissolved in 10 mL of an aqueous solution with 50% ethanol content. Next, spread 100 mg of the vinyl-functionalized hybrid material on...
Embodiment 2
[0023] Example 2 The glutathione functionalized hybrid material is used for the separation and enrichment of glycopeptides.
[0024] Preparation of glutathione-functionalized hybrid materials:
[0025] 1) Preparation of vinyl-functionalized hybrid materials: first weigh 50 mg of dodecylamine and 20 mg of CTAB into a centrifuge tube, add 175 μL of ethanol and 125 μL of water, ultrasonically dissolve to form a homogeneous solution, and then add 100 mg of Tetraethoxysilane and 100mg 7-octenyltrimethoxysilane were placed in a water bath at 40°C for 24 hours; the hybrid material was washed three times with ethanol / water solution (1 / 1, v / v) , Vacuum drying at 60°C for 12h to obtain a vinyl-functionalized hybrid material.
[0026] 2) Preparation of glutathione-modified hybrid material: First, 20 mg of glutathione and 2 mg of photoinitiator DMPA were dissolved in 10 mL of an aqueous solution with 50% ethanol content. Next, spread 100 mg of the vinyl-functionalized hybrid material on...
Embodiment example 1
[0030] Implementation case 1 prepares the infrared image of the hybrid material such as figure 2 As shown, in the infrared spectra of the functional monomers tetraethoxysilane and 7-octenyltrimethoxysilane, the wave numbers are 2974 and 2889cm -1The absorption peak at Si-O-C 2 h 5 The C-H stretching vibration in . And the wave numbers are 1391, 1296cm -1 The absorption peak at -CH 3 The symmetric bending vibration of C-H in the middle. 1100 and 1073cm -1 The absorption peak at Si-O-C 2 h 5 Si-O stretching vibration in . After the free radical reaction is completed, a 1602cm -1 The C=C stretching vibration peak at 1391, 1296cm -1 attributable to -CH 3 The symmetrical bending vibration of C-H in , while 1100 and 1073cm -1 attributable to Si-O-C 2 h 5 The Si-O stretching vibration doublet in the -1 Si-O broad peak substitution of Si-O-Si. This shows that the C=C functional group has been introduced into the synthesized hybrid material, which provides favorable co...
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