Multistage separating micro-fluidic chip for cells

Abstract

The invention discloses a multistage separating micro-fluidic chip for cells. The multistage sorting micro-fluidic chip consists of an upper cover plate, a filter layer, a magnetic separating layer, aspiral separating layer, and a lower cover plate which are overlapped, wherein the magnetic separating layer consists of a separating layer and a diversion layer; third sample liquid inlets, a focussine flow channel, an abrupt expansion structure and a magnetic separating flow channel are arranged on the separating layer; a magnetic bead outlet and a first cell outlet are formed in the magneticseparating flow channel; a diversion layer inlet, a diversion layer flow channel and a diversion layer outlet are formed in the diversion layer; a fourth sample liquid inlet, a spiral flow channel, asecond cell outlet and a third cell outlet are formed in the spiral separating layer; and the diversion layer outlet communicates with the fourth sample liquid inlet. The chip integrates magnetic andinertia spiral separating two-stage flow channels, and sufficiently adopts high-flux advantages of inertia spiral separating and high-precision advantages of magnetic separating, so that high-flux high-purity separating on different cells can be realized, the cost is low, the operation is simple, and integrated miniaturization is easy to realize.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip for multistage sorting of cells. Background technique [0002] Microfluidic technology precisely manipulates microliter and milliliter samples through micron-scale flow channels. Thanks to its characteristic size that just matches the cell size, these microfluidic devices have the inherent advantages of low sample consumption and high control accuracy. The potential for sorting applications is huge. At present, the sorting technology is mainly divided into active and passive. The active cell sorting technology needs to use external electric, sound, light, magnetic and other physical fields to exert force on the cells to achieve cell sorting. The sorting accuracy is high, but its The cost is high, and it is difficult to achieve high-throughput sorting; passive cell sorting refers to not relying on external physical fields, but only relying on the physical structure of th...

AI Technical Summary

Problems solved by technology

At present, the sorting technology is mainly divided into active and passive. The active cell sorting technology needs to use external electric, sound, light, magnetic and other physical fields to exert force on the cells to achieve cell sorting. The sorting accuracy is high, but its The cost is high, and it is difficult to achieve high-throughput sorting; passive cell sorting refers to not relying on external physical fields, but only relying on the physical structure of the microfluidic chip itself or the fluid dynamics generated by the interaction between cells in the flow channel, so as to achieve cell separation. The sorting method of position differentiation in the flow channel is mainly based on physical properties such as particle density, shape, and deformability. Passive sorting meets the requirements of high-throughput sorting, and has a simple structure and low cost. Low selection accuracy and low recovery purity

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