A strain of Bacillus brevis for controlling root-knot nematode and its application
A technology of Bacillus brevis, root knot nematode, applied in the field of microbial pesticides and microbial inoculants, it can solve the problems of unreported application, and achieve the effect of low cost of use and safety for humans and animals
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Embodiment 1
[0022] Cultivation of DL01 strain and preparation of bacterial agent
[0023] 1) Cultivation of DL01 strain slant seeds in a test tube: the strain was inoculated on the slant surface of LB medium, cultured at 28° C. for 1 day to obtain slant seeds.
[0024] 2) Liquid seed culture of the DL01 strain: inoculate the slant seeds into a conical flask containing beef extract peptone liquid medium, culture on a shaker at 28° C. and 150-180 rpm for 2 days to obtain liquid seeds.
[0025] 3) Large-scale cultivation of DL01 strain fermenter: the liquid seeds are inserted into the beef extract peptone liquid medium in the fermenter at a ratio of 3% (V / V), and the culture conditions in the 1000 liter fermenter are controlled at: temperature 32 ℃, stirring speed 150rpm, fermentation time 72 hours.
[0026] 4) Preparation of DL01 microbial agent: the microbial cells and their metabolites obtained through fermenter cultivation are mixed with an appropriate amount of diatomaceous earth, drie...
Embodiment 2
[0028] Cultivation of DL01 strain and preparation of bacterial agent
[0029] 1) Cultivation of DL01 strain slant seeds in a test tube: the strain was inoculated on the slant surface of LB medium, and the culture condition was 30° C. for 2 days to obtain slant seeds.
[0030] 2) Liquid seed culture of the DL01 strain: Inoculate the slanted seeds into a triangular flask filled with beef extract-peptone liquid medium, and cultivate them at 30° C. for 2 days to obtain liquid seeds.
[0031] 3) Large-scale cultivation of DL01 strain fermenter: the liquid seeds are inserted into the beef extract peptone liquid medium in the fermenter at a ratio of 3% (V / V), and the culture conditions in the 1000 liter fermenter are controlled at: temperature 32 ℃, stirring speed 150rpm, fermentation time 72 hours.
[0032] 4) Preparation of DL01 microbial agent: the microbial cells and their metabolites obtained through fermenter cultivation are mixed with an appropriate amount of diatomaceous ear...
Embodiment 3
[0034] Cultivation of DL01 strain and preparation of bacterial agent
[0035] 1) DL01 strain test tube slant seed culture: the strain was inoculated on the LB medium slant, cultivated at 32°C for 3 days to obtain slant seeds;
[0036] 2) Liquid seed culture of DL01 strain: inoculate the slanted seeds into a triangular flask containing beef extract peptone liquid medium, and culture on a shaker at 32°C and 150-180rpm for 3 days to obtain liquid seeds;
[0037] 3) Large-scale cultivation of the DL01 strain fermenter: the liquid seeds are inserted into the beef extract peptone liquid medium in the 1000-liter fermenter at a ratio of 3% by volume, and the culture conditions are controlled at: temperature 32 ° C, stirring speed 150 rpm, fermentation Time 72 hours.
[0038] 4) Preparation of DL01 bacterial agent: the microbial cells and their metabolites obtained by fermenter cultivation are mixed with 2 times the volume of diatomaceous earth, then dried below 65°C or air-dried unti...
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