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Antibody for specifically binding coronavirus, and antigen binding fragment of antibody

A technology that combines fragments and coronaviruses, applied in the direction of antibodies, antiviral agents, antiviral immunoglobulins, etc., can solve problems such as loss of protective effect of mutant viruses

Active Publication Date: 2020-10-20
超非凡(上海)医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the non-conserved region recognized by these antibodies is mutated to produce a new circulating strain, the antibody will lose its protective effect against the mutant virus

Method used

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  • Antibody for specifically binding coronavirus, and antigen binding fragment of antibody
  • Antibody for specifically binding coronavirus, and antigen binding fragment of antibody
  • Antibody for specifically binding coronavirus, and antigen binding fragment of antibody

Examples

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Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Screening and detection of antibodies specifically binding to coronavirus

[0061] The inventor conducted a pseudovirus assay on the plasma of patients with novel coronavirus pneumonia (followed up two weeks after recovery and discharge) admitted to the inventor's unit (Shanghai Public Health Clinical Center) from January 20, 2020 to February 26, 2020. And experimental screening, it was found that the serum of one of the mild patients had a strong neutralizing activity against the SARS-CoV-2 pseudovirus. With the written consent of the ethics committee of the inventor's unit and the patient himself, his peripheral blood was drawn for research.

[0062] 1. Sorting of peripheral blood memory B cells

[0063] 1) Separation of peripheral blood lymphocytes: extract the convalescent peripheral blood of the above-mentioned patients and mix it with an equal amount of normal saline, then use Lymphoprep (Stemcell Technologies, Cat. No. 07851) to separate peripheral blo...

Embodiment 2

[0094] This example describes a method that can be used to treat diseases caused by coronaviruses including SARS-CoV-2 by administering the coronavirus-specific monoclonal antibodies of the present application.

[0095] Although specific methods, dosages and modes of administration have been provided, it will be understood by those skilled in the art that changes may be made without substantially affecting the treatment. Based on the teachings disclosed herein, coronavirus infection can be treated or prevented by administering a therapeutically effective amount of one or more of the antibodies described herein, thereby reducing or eliminating coronavirus infection.

[0096] The specific application method is as follows:

[0097] 1) Screening of subjects: First, subjects are screened to determine whether they are infected with coronaviruses such as SARS-CoV-2. The method for screening SARS-CoV-2 infection can use respiratory specimen nucleic acid detection combined with clinic...

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Abstract

The invention relates to an antibody for specifically binding a coronavirus, or an antigen binding fragment of the antibody, a nucleic acid molecule for encoding the antibody or the antigen binding fragment, a carrier containing the nucleic acid molecule, a host cell containing the carrier, application of an aspect of the antibody or the antigen binding fragment for preparing a medicine capable oftreating or preventing diseases caused by the coronavirus, and the application on an aspect of product detection. The antibody for specifically binding the coronavirus, and the antigen binding fragment of the antibody have broad-spectrum neutralization capacity on SARS-CoV-2, SARS-CoV, SARS-like coronaviruses and the like, and have a good clinical application prospect in the future.

Description

technical field [0001] The present invention relates to an antibody or its antigen-binding fragment specifically binding to coronavirus, a nucleic acid molecule encoding the antibody or its antigen-binding fragment, a vector comprising the nucleic acid molecule, a host cell comprising the vector, and the antibody or its antigen The application of combining fragments to prepare drugs for treating or preventing diseases caused by coronaviruses, as well as the application in testing products, belongs to the field of biomedicine. Background technique [0002] Novel coronavirus pneumonia (2019-nCOV) is an acute respiratory infectious disease caused by a new type of coronavirus, SARS-COV-2. The virus is highly transmissible and can be transmitted through the respiratory tract, contact and other channels. Since the outbreak in December 2019, it has spread to all parts of the world, forming a worldwide pandemic. As of July 1, 2020, the SARS-CoV-2 coronavirus has caused more than 10...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/10C12N15/13C12N15/70C12N1/21A61K39/42A61P31/14G01N33/577G01N33/569
CPCC07K16/10C12N15/70A61P31/14G01N33/577G01N33/56983C07K2317/565C07K2317/56C07K2317/76C07K2317/92A61K2039/505G01N2333/165G01N2469/10
Inventor 黄竞荷吴凡刘梅
Owner 超非凡(上海)医疗科技有限公司