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Non-targeted lipidosome identification method for goat milk production places

A goat milk, lipidome technology, used in molecular entity identification, measurement devices, instruments, etc.

Pending Publication Date: 2020-10-27
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies on the identification of different origins of milk based on lipidomics have not been reported

Method used

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  • Non-targeted lipidosome identification method for goat milk production places
  • Non-targeted lipidosome identification method for goat milk production places

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Establishment and application of non-targeted lipid group identification method for goat milk origin

[0040] S1. Sample collection;

[0041] Focusing on the main producing areas of goat milk, 31 mature goat milk samples were collected from 3 farms in Yangling District of Shaanxi Province, Qingdao of Shandong Province and Luliang County of Yunnan Province.

[0042] S2. Sample pretreatment;

[0043] Mix the goat milk sample with pure water and a mixed organic solvent and centrifuge; remove the organic solution in the lower layer and dry it for later use. The goat milk sample: ultrapure water: the volume ratio of the mixed organic solvent is 1:5:15; the mixed organic solvent is composed of chloroform and methanol in a volume ratio of 2:1.

[0044] S3. analyzing the lipid composition of the sample to obtain lipid composition data;

[0045] All samples were tested on the Tsinghua Metabolomics Platform. Detected by UPLC-Q-Extractive Orbitrap Mass of Thermo Fish...

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Abstract

The invention discloses a non-targeted lipidosome identification method for goat milk production places. The method comprises the following steps of: S1, collecting samples; S2, pre-treating the samples; S3, analyzing the lipid compositions of the samples to obtain lipid composition data; S4, performing multivariable statistical analysis processing on the obtained lipid composition data; S5, establishing a Fisher model to obtain the characteristic lipid compositions of the goat milk from different production places; S6, distinguishing the goat milk from different production places by utilizingthe difference lipid compositions. By adopting the method disclosed by the invention, 15 lipid subclasses and 756 lipid molecules are identified in total, wherein five lipid subclasses and 51 lipid molecules have significant differences in different production places, goat milk production places established based on the potential indexes are gradually discriminated and analyzed, six lipid molecules are screened out, a discrimination model is established, and the initial and cross validation correct discrimination rates of the model are both 100%.

Description

technical field [0001] The invention relates to a method for identifying goat milk production areas, in particular to a non-targeted lipid group identification method for goat milk production areas, and belongs to the field of identification of goat milk production areas. Background technique [0002] Goat milk is a traditional Chinese specialty milk. China is an important sheep-raising country in the world, and it is also one of the countries with the largest number of dairy goats in the world. In 2017, China's goat milk production reached 238,000 tons, and the number of dairy goats exceeded 1.29 million. China has a long history of dairy goat breeding. Compared with cow's milk produced under the same conditions, goat's milk contains higher content of short-chain and medium-chain fatty acids, unsaturated fatty acids, omega-6FA, omega-3FA, DHA and EPA. Up to now, there are several famous dairy goat breeding areas in China, such as Fuping in Shaanxi and Laoshan in Shandong...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86G16C20/20
CPCG01N30/88G01N30/8693G16C20/20
Inventor 刘宏艳秦玉昌张军民赵青余郭晓青
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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