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A culture medium for clustering buds of Peucedanum pectoralis with bud tips as explants

A medium and explant technology, applied in the field of Qianhu tissue culture, can solve the problems of low differentiation efficiency and easy infection with viruses, and achieve the effects of improving production efficiency, increasing induction rate and low virus infection rate

Active Publication Date: 2022-04-12
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial propagation of Peucedanum mainly adopts seeding and tissue culture. The existing tissue culture technology of Peucedanum mostly uses leaves, petioles and stems as explants, which not only has low induction and differentiation efficiency, but also is prone to virus infection.

Method used

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  • A culture medium for clustering buds of Peucedanum pectoralis with bud tips as explants
  • A culture medium for clustering buds of Peucedanum pectoralis with bud tips as explants
  • A culture medium for clustering buds of Peucedanum pectoralis with bud tips as explants

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Experimental program
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Embodiment 1

[0015] Preparing Peucedanum bushy shoot medium, the composition is as follows: every 1000mL described medium contains 6-benzylaminoadenine 1.5mg, kinetin 0.5mg, indole acetic acid 0.2mg, coconut milk 120mg, agar powder 3g, sucrose 28g, remaining The amount is MS medium.

Embodiment 2

[0017] Preparing Peucedanum bushy shoot medium, the composition is as follows: every 1000mL described medium contains 6-benzylaminoadenine 2.5mg, kinetin 1.5mg, indole acetic acid 0.4mg, coconut milk 160mg, agar powder 5g, sucrose 34g, remaining The amount is MS medium.

Embodiment 3

[0019] Preparing Peucedanum cluster shoot medium, the composition is as follows: every 1000mL described medium contains 6-benzylaminoadenine 1.8mg, kinetin 0.8mg, indole acetic acid 0.35mg, coconut milk 130mg, agar powder 4g, sucrose 31g, remaining The amount is MS medium.

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Abstract

The invention discloses a culture medium for the clustering buds of Peucedanum with bud tips as explants. Every 1000 mL of the culture medium contains 1.5-2.5 mg of 6-benzylaminoadenine, 0.5-1.5 mg of kinetin, and 0.2 mg of indole acetic acid. ‑0.4mg, coconut milk 120‑160mg, agar powder 3‑5g, sucrose 28‑34g, and the rest is the basic medium. The Peucedanum clustered bud culture medium using bud tips as explants can greatly increase the induction rate of Peucedaneum clustered shoots induced by using bud tips as explants, improve production efficiency, and save production costs.

Description

technical field [0001] The invention relates to the technical field of Peucedaneum tissue culture, in particular to a culture medium for the clustering buds of Peucedanum pectoralis with bud tips as explants. Background technique [0002] Peucedanum is a perennial herb of Umbelliferae, its roots are irregularly cylindrical, conical or spindle-shaped, and the surface is dark brown or grayish yellow. Peucedanum is a commonly used traditional Chinese medicinal material. The dried root is used as medicine. It has the effect of reducing qi and reducing phlegm, dispelling wind and clearing heat. [0003] At present, the artificial propagation of Peucedanum mainly adopts seeding and tissue culture methods. The existing tissue culture technology of Peucedanum mostly uses leaves, petioles and stems as explants, which not only has low induction and differentiation efficiency, but also is prone to virus infection. Contents of the invention [0004] Based on the technical problems ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 黄和平汪电雷黄鹏彭华胜
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE