Water improvement micro-ecological preparation

A micro-ecological preparation and water body improvement technology, applied in the direction of microorganisms, microorganism-based methods, biological water/sewage treatment, etc., can solve problems such as water quality deterioration, fish death, fishery and aquaculture hazards, etc.

Inactive Publication Date: 2020-10-30
TIANJIN RUANYIN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The green tide causes the water quality to deteriorate, and in severe cases, the oxygen in the water will be exhausted, causing the death of fish and causing serious harm to the fishery and aquaculture industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] (2) Preparation of seed solution

[0025] According to the ratio (volume percentage) of 0.05%-0.5%, add the inoculum prepared in step (1) in the 250ml Erlenmeyer flask containing beef extract peptone liquid culture, 30 ℃, 200 rpm / min shaker culture 21-24h, obtain Bacterial liquid containing Bacillus subtilis at a concentration not less than 5×10 9 CFU / ml. The seed solution preparation method of Rhodopseudomonas palustris and Brevibacillus laterosporus is the same as that of Bacillus subtilis. Saccharomyces cerevisiae (Sacchuromyces cerevisiue) was added to the culture medium containing YPD for 48-72h, and the rest of the steps were the same as above;

[0026] (3) Fermentation culture

[0027] A: The bacterial seed solution obtained in step (2) is inserted into the broth medium according to the ratio of 1-10% (volume percentage), and then cultivated at 28-34°C and the speed of seeding bed is 170-210rpm / min 22-25 hours to obtain individual strain preparations;

[002...

specific Embodiment approach 2

[0031] (1) Preservation and activation of strains

[0032] Bacillus subtilis was stored at 4°C in TSB medium containing 15% glycerol. The formula of TSB medium: Weigh 30g of Tryptic Soy Broth (TSB) medium powder and add it to 1000ml of distilled water, mix well. Ready to use after autoclaving. Use a micropipette to pipette 10μl of the bacterial solution into 3-5ml of liquid beef extract peptone medium, pH ≈ 7.0-7.2; culture on a shaker at 200rmp / min, 30°C, 18-20 hours. The preservation and cultivation methods of Rhodopseudomonas palustris and Brevibacillus laterosporus are the same as those of Bacillus subtilis;

[0033] Saccharomyces cerevisiae was maintained at 4°C in YPD medium containing 15% glycerol. The formula of YPD substratum: take by weighing 49g YcastPeptoncDextrosc Agar (YPD) substratum powder and add 1000ml distilled water, mix, after high-pressure fire bacteria, for subsequent use;

[0034] (2) Preparation of seed solution

[0035] According to the ratio (vo...

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PUM

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Abstract

The invention discloses a water improvement micro-ecological preparation. The water improvement micro-ecological preparation is prepared from bacillus licheniformis, rhodopseudomonas palustris, bacillus laterosporus, saccharomyces cerevisiae, rhodopseudomonas palustris, rhodopseudomonas palustris, bacillus laterosporus, saccharomyces cerevisiae, saccharomyces cerevisiae, rhodopseudomonas palustris, bacillus laterosporus and saccharomyces cerevisiae according to a content ratio of 1: 1.5: 1.5: 1. The invention develops a novel preparation for inhibiting the growth of blue-green algae for surface water and aquaculture, and can effectively improve the quality of water and remove peculiar smell of the water.

Description

technical field [0001] The invention relates to a novel microbial preparation, which can effectively inhibit the growth of cyanobacteria, improve the quality of water body and remove the peculiar smell of water body. Background technique [0002] Cyanobacteria are single-celled prokaryotes, also known as blue-green algae and cyanobacteria, but they are not bacteria, nor are they green algae. Cyanobacteria is a general term for a class of algae whose hallmark is a single cell without a nucleus wrapped in a nuclear membrane. Is a class of Gram-negative bacteria that contain pigments and are capable of photosynthesis. [0003] In some nutrient-rich water bodies (the water contains nitrogen and phosphorus), blue-green algae often multiply in large numbers in summer, and form a layer of blue-green and fishy-smelling foam on the water surface, called "water bloom", large-scale blue-green algae outbreaks , known as the "green tide" (corresponding to the red tide that occurs in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/125C12R1/01C12R1/865
CPCC12N1/20C12N1/18C02F3/34C02F3/347
Inventor 焦华周建娜
Owner TIANJIN RUANYIN TECH CO LTD
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