A kind of detection method and application of goat ppp3ca gene indel marker
A gene and genotype technology, applied in the field of detection of PPP3CA gene InDel markers related to the litter size of goats, can solve the problems such as DNA markers that have not yet been seen, and achieve the advantages of speeding up the process of marker-assisted selection breeding, low cost, and simple detection methods. Effect
Active Publication Date: 2022-04-26
NORTHWEST A & F UNIV
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Problems solved by technology
InDel research is currently mostly focused on the genome research of humans and various crops (such as rice and corn, etc.), and the mining and application of InDel markers for functional genes of ruminant livestock is urgently needed
So far, there is no report on the DNA markers related to goat PPP3CA gene and litter size marker-assisted selection
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[0023] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are explanations of the present invention, rather than limiting the protection scope of the present invention.
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The invention discloses a detection method and application of goat PPP3CA gene InDel marker. The genotype of the InDel site at position NC_030813.1:g.16472‑16491 of the goat PPP3CA gene was identified by PCR amplification and agarose gel electrophoresis using the whole genome DNA of the goat to be tested as a template. According to the correlation analysis results of different genotypes of InDel locus of PPP3CA gene and litter size of northern Shaanxi white cashmere goats, it was determined that the genotype of InDel locus of PPP3CA gene could be used as a DNA marker to increase litter size of goats. The invention quickly and accurately detects InDel markers closely related to goat litter size, can quickly establish goat dominant genetic resource groups, thereby accelerating the marker-assisted selection breeding process of goat reproductive traits.
Description
technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion polymorphism (InDel), in particular to the rapid and accurate detection of the PPP3CA gene InDel marker related to goat litter size. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of the molecular breeding technology system, marker-assisted selection (MAS) breeding technology selects traits based on DNA markers that are significantly related to genetic traits. The direct selection of the type can improve the orientation of the breeding target, and it has advantages in early selection, evaluation and selection of non-destructive traits, and improvement of backcross breeding efficiency. Finding important functional genes, screening important gene...
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IPC IPC(8): C12Q1/6888C12Q1/686
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124C12Q2565/125
Inventor 白洋洋王新雨蓝贤勇潘传英陈宏雷初朝黄永震朱海鲸董书伟屈雷
Owner NORTHWEST A & F UNIV