Detection method of goat PPP3CA gene InDel marker and application of goat PPP3CA gene InDel marker

Abstract

The invention discloses a detection method of a goat PPP3CA gene InDel marker and application of the goat PPP3CA gene InDel marker. The genotype of the InDel site at the site NC _ 030813.1: g.16472-16491 of the goat PPP3CA gene is identified by taking the whole genome DNA of the goat to be detected as a template through PCR (Polymerase Chain Reaction) amplification and agarose gel electrophoresis.According to correlation analysis results of different genotypes of the InDel site of the PPP3CA gene and the litter size of Shaanbei white cashmere goats, it is determined that the genotype of the InDel site of the PPP3CA gene can be used as a DNA marker for increasing the litter size of the goats. By rapidly and accurately detecting the InDel marker closely related to the litter size of the goats, a goat dominant genetic resource group can be rapidly established, so that the marker-assisted selective breeding process of goat reproductive traits is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion polymorphism (InDel), in particular to the rapid and accurate detection of the PPP3CA gene InDel marker related to goat litter size. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of the molecular breeding technology system, marker-assisted selection (MAS) breeding technology selects traits based on DNA markers that are significantly related to genetic traits. The direct selection of the type can improve the orientation of the breeding target, and it has advantages in early selection, evaluation and selection of non-destructive traits, and improvement of backcross breeding efficiency. Finding important functional genes, screening important gene...

AI Technical Summary

Problems solved by technology

InDel research is currently mostly focused on the genome research of humans and various crops (such as rice and corn, etc.), and the mining and application of InDel markers for functional genes of ruminant livestock is urgently needed

So far, there is no report on the DNA markers related to goat PPP3CA gene and litter size marker-assisted selection

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