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Goat AKAP12 gene InDel marker detection method and application thereof

A detection method and gene technology, which is applied in the field of detection of the AKAP12 gene InDel marker related to the body length traits of goats, can solve problems such as DNA markers that have not yet been seen, and achieve the effects of accelerated breeding speed, low cost, and simple detection methods

Active Publication Date: 2020-10-02
YULIN UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

InDel research is mostly concentrated in the genome research of humans and various crops (such as rice and corn, etc.), and the mining and application of InDel markers for functional genes of ruminant livestock is urgently needed.
There are no reports of DNA markers related to marker-assisted selection of growth traits in the goat AKAP12 gene

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  • Goat AKAP12 gene InDel marker detection method and application thereof
  • Goat AKAP12 gene InDel marker detection method and application thereof
  • Goat AKAP12 gene InDel marker detection method and application thereof

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Embodiment Construction

[0023] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are explanations of the present invention, rather than limiting the protection scope of the present invention.

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Abstract

The invention discloses a goat AKAP12 gene InDel marker detection method and application thereof. To-be-detected goat whole-genome DNA is taken as a template, and genotypes of InDel sites in NC_030816.1:g.110266-110278 positions of a goat AKAP12 gene are identified through PCR amplification and agarose gel electrophoresis. According to an association analysis result of the goat AKAP12 gene InDel sites and body length of Northern Shaanxi white cashmere goats, the genotypes of the AKAP12 gene InDel sites are determined to be taken as DNA markers for increasing goat body length. By rapid and accurate detection for the InDel sites closely related to goat body length characters, a goat dominant genetic resource group can be rapidly established, so that a marker assisted selection breeding process of the goat growth characters is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion polymorphism (InDel), in particular to the rapid and accurate detection of the AKAP12 gene InDel marker related to the body length traits of goats. Background technique [0002] Animal breeding technology mainly includes conventional breeding technology based on phenotype and phenotypic value and molecular breeding technology based on DNA polymorphism. As an important part of the molecular breeding technology system, marker-assisted selection (MAS) breeding technology selects traits based on DNA markers that are significantly related to genetic traits. The direct selection of the type can improve the orientation of the breeding goal. The method has advantages in early selection, evaluation and selection of non-destructive traits, and improvement of backcross breeding efficiency. Finding important functional genes, screen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2565/125Y02P60/87
Inventor 宋晓越袁荣荣白洋洋屈雷朱海鲸蓝贤勇
Owner YULIN UNIV