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ssc-miR-4334-5p used for preparing kit for detecting foot-and-mouth disease infection and medicine for treating foot-and-mouth disease

A kit, technology of foot-and-mouth disease virus, applied in the field of biomedicine

Inactive Publication Date: 2020-10-30
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there are no miRNA drugs based on ssc-miR-4334-5p related for FMDV diagnosis and treatment

Method used

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  • ssc-miR-4334-5p used for preparing kit for detecting foot-and-mouth disease infection and medicine for treating foot-and-mouth disease
  • ssc-miR-4334-5p used for preparing kit for detecting foot-and-mouth disease infection and medicine for treating foot-and-mouth disease
  • ssc-miR-4334-5p used for preparing kit for detecting foot-and-mouth disease infection and medicine for treating foot-and-mouth disease

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the drawings.

[0023] 1. The relative expression results of miR-4334-5p:

[0024] Ssc-miR-4334-5p (MIMAT0017966; CCCUGGAGUGACGGGGGUG) The mimics and inhibitors used in the experiment and the corresponding scramble sequences are as follows:

[0025] miR-4334-5p mimics:

[0026] sense 5’-CCCUGGAGUGACGGGGGUG-3’,

[0027] antisense 5’-CCCCCGUCACUCCAGGGUU-3’.

[0028] scrambled negative-control:

[0029] sense 5’-GGUGGCGCGUCGAGCGUGA-3’,

[0030] antisense 5’-ACGCUCGACGCGCCACCUU-3’.

[0031] miR-4334-5p inhibitor 5’-CACCCCCGUCACUCCAGGG-3’;

[0032] scrambled negative-control 5’-CCACGGUCGACACCGCCUC-3’.

[0033] The primer sequence of miR-4334-5p-F used for qRT-PCR quantification is: 5’-CCCTGGAGTGACGGGGGTG-3’, set miR-16-F

[0034] (5'-GCAGTAGCAGCACGTA-3') is used as the internal reference, and the downstream primer is the universal primer provided in the kit (miScript SYBR GreenPCR kit).

[0035] Cell preparation:

[0036...

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Abstract

The invention relates to ssc-miR-4334-5p used for preparing a kit for detecting foot-and-mouth disease virus infection and a medicine for treating foot-and-mouth disease virus infection. The inventiondiscloses the kit for detecting foot-and-mouth disease virus infection. The kit is used for detecting the expression level of ssc-miR-4334-5p. The kit comprises a fluorescent quantitative PCR primerfor detecting ssc-miR-4334-5p. The fluorescent quantitative PCR primer is prepared from a base sequence miR-4334-5p-F: 5'-CCCTGGAGTGACGGGGGTG-3'. The invention discloses a medicine for treating foot-and-mouth disease virus infection, and the medicine comprises an ssc-miR-4334-5p inhibitor, and the ssc-miR-4334-5p inhibitor comprises mRNA with a sequence of 5'-CACCCCCGUCACUCCAGGG-3'. The kit has the beneficial effects that the expression level of ssc-miR-4334-5p after FMDV infection is detected through fluorescent quantitative PCR, and exploration of a potential non-coding RNA target for diagnosing virus infection is facilitated. According to the invention, the effect of ssc-miR-4334-5p in inhibition of FMDV is proved for the first time, and development of a novel therapeutic miRNA drug forresisting virus infection is facilitated.

Description

Technical field [0001] The invention belongs to the field of biomedicine and relates to the application of ssc-miR-4334-5p. Background technique [0002] Foot-and-mouth disease (FMD) is caused by foot-and-mouth disease virus (FMDV) infection. The disease mainly occurs in cattle, sheep, goats, pigs and other cloven-hoofed animals. It is characterized by contact with the hooves, mouth, and nose of infected animals. Blisters are formed on the skin of the disease, and the outbreak of the disease can cause serious economic losses. The virus is considered to be one of the most infectious viruses known. During FMDV infection, the expression of type I interferon (IFN) and pro-inflammatory cytokines is up-regulated by activation of nuclear transcription factor κB (NF-κB) and IFN regulatory factors 3 and 7 (IRF3 / 7), thereby forming an antiviral state . [0003] MicroRNA (miRNA) is a type of single-stranded non-coding RNA with a length of about 22 nt encoded by endogenous genes. It is prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851A61K31/7105A61P31/14C12R1/93
CPCA61K31/7105A61P31/14C12Q1/6851C12Q1/701C12Q2600/158C12Q2600/178C12Q2563/107C12Q2545/101C12Q2531/113
Inventor 孙跃峰任亭亭王妍鳕
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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