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Method for rapidly evaluating salt tolerance of willow varieties based on resistance value

A technology of resistance value and salt tolerance is applied in the field of rapid identification and evaluation of salt tolerance of willow varieties, which can solve problems such as large influence

Active Publication Date: 2020-10-30
NANTONG UNIVERSITY
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Problems solved by technology

[0002] Salt tolerance is the main character of plant stress resistance. The traditional salt tolerance identification method is to plant the plants on saline soil and evaluate by measuring the survival rate and growth status of the plants. It usually takes more than one year to evaluate the plants to be tested. Variety's salt tolerance ability, and is greatly affected by the external environment

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  • Method for rapidly evaluating salt tolerance of willow varieties based on resistance value

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Embodiment Construction

[0031] The present invention will be further explained below.

[0032] (1) Material collection and processing

[0033] Get the branches of the annual thickness 3-5mm of the willow variety to be measured, each kind of processing gets 10, every 8cm is long, measures the middle thickness of every with vernier caliper, and the initial water content and the initial resistance value of branch; Measure three times and take the average value. Then dip the branches into the NaCl solution with a concentration of 100mM / L, take them out at intervals of 2, 4, 6, and 8 hours, and measure the resistance value each time. At 192h (8d), the water content, the number of lenticels and black holes and the electrical conductivity of the branches were measured.

[0034] (2) Determination of water content

[0035] Using the YHT01 moisture tester produced by Shenzhen Yuanhengtong Technology Co., Ltd., in the "White PoplarKeruing" test mode, insert two probe heads into the two ends of the branch 1mm...

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Abstract

The invention discloses a method for rapidly evaluating salt tolerance of willow varieties based on a resistance value, which comprises the steps of taking a plurality of annual branches with the thickness of 3-5mm of each willow variety to be tested, wherein the branches are the same in length; measuring the intermediate thickness of each branch, the initial water content of the branch and the initial resistance value; soaking the branches into a NaCl solution, taking out the branches when the branches are soaked for 2 hours, 4 hours, 6 hours and 8 hours, measuring the resistance value, and taking out the branches when the branches are soaked for 192 hours, and measuring the water content, the number of lenticel black holes and the conductivity value of the branches; and grading by adopting a willow salt tolerance grading evaluation model based on the initial resistance value. The method is convenient to operate, can quickly evaluate the salt tolerance of the willow variety, is not influenced by the external environment, and can evaluate the salt tolerance of the to-be-tested sample within 10 days.

Description

technical field [0001] The patent of the invention belongs to the field of stress resistance of plants, and specifically relates to a rapid identification and evaluation method for the salt-tolerant ability of willow varieties. Background technique [0002] Salt tolerance is the main character of plant stress resistance. The traditional salt tolerance identification method is to plant the plants on saline soil and evaluate by measuring the survival rate and growth status of the plants. It usually takes more than one year to evaluate the plants to be tested. The salt tolerance of varieties is greatly affected by the external environment. Willow is the general name of Salicaceae Salicaceae and Salix genus. It is an important stress-resistant tree species and plays an important role in soil heavy metal restoration, saline-alkali land greening, biofuels, etc. Many species have good salt tolerance, and some species The salt tolerance of the plant has exceeded 4‰ (extreme salt to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/04G01N33/00G01N21/84G01N27/06
CPCG01N21/84G01N27/041G01N27/06G01N33/0098G01N2021/8466
Inventor 张健刘国元陈艳红江钰娜余春梅钟非连博琳柯勇超郭安芳朱星兆刘昱
Owner NANTONG UNIVERSITY