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Application of advanced effect T cell subset in preparation of kit for assisting in evaluating condition degree of aplastic anemia

A technology of aplastic and cell subgroups, applied in the field of biomedicine, can solve the problems that have not yet been seen in the characteristic changes of T cells in AA patients

Pending Publication Date: 2020-10-30
JINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports on the characteristic changes of late effector T cells in AA patients

Method used

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  • Application of advanced effect T cell subset in preparation of kit for assisting in evaluating condition degree of aplastic anemia
  • Application of advanced effect T cell subset in preparation of kit for assisting in evaluating condition degree of aplastic anemia
  • Application of advanced effect T cell subset in preparation of kit for assisting in evaluating condition degree of aplastic anemia

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Experimental program
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Effect test

Embodiment 1

[0066] (1) Blood was collected on the premise of signing the informed consent form with AA patients, and all specimens were taken from fasting intravenous EDTA anticoagulation in the morning. Peripheral blood samples were collected from a total of 43 AA patients, including 8 NSAA, 21 SAA and 14 VSAA. At the same time, 43 healthy samples were collected, and this part of the research protocol has been approved by the ethics committee of this unit. At the same time, clinical data such as hemoglobin (HB), platelet (PLT) and absolute neutrophil value (ANC) of AA patients were collected (as shown in Table 1).

[0067] Table 1 Clinical data of patients with AA

[0068]

[0069]

[0070] (2) The collected peripheral blood of healthy people and AA patients was split into erythrocyte lysate. Mix 2 mL of erythrocyte lysate per 200 μL of peripheral blood, and lyse at room temperature for 10 min. During the lysing period, gently pipette and mix once with a pipette, then centrifuge ...

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Abstract

The invention discloses application of an advanced effect T cell subset in preparation of a kit for assisting in evaluating the disease degree of aplastic anemia. That the ratio of CD4 + CD45RO + CD27-T cells in peripheral blood of an AA patient is significantly reduced is found for the first time; the VSAA and the SAA of the AA patient are obviously lower than those of healthy people; only the proportion of CD4 + CD45RO + CD27-T cells in peripheral blood of VSAA and SAA patients is significantly lower than the characteristic change of NSAA patients, and the characteristic change can be used as one of laboratory immunity related detection index for assisting evaluating the illness severity of AA patients. Meanwhile, important reference data are provided for clinical selection of treatmentstrategies for AA patients with different disease degrees in the future.

Description

technical field [0001] The invention belongs to the field of biomedicine, and particularly relates to the application of a late effector T cell subset in the preparation of a kit for assisting assessment of the severity of aplastic anemia. Background technique [0002] Aplastic anemia (AA) is a hematologic disease characterized by decreased bone marrow hematopoietic cell proliferation and pancytopenia. The annual incidence of AA in my country is much higher than that in Europe and the United States. At present, the clinical diagnosis of AA is mainly based on the patient's peripheral blood routine examination, multi-site bone marrow aspiration smear analysis, bone marrow biopsy, and exclusion of congenital and pancytopenia and bone marrow hypoplasia. Diagnosis is made on the basis of other diseases, and AA is divided into non-severe aplastic anemia (NSAA) and severe aplastic anemia (SAA) according to the degree of bone marrow cell proliferation and blood routine examination. a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/577G01N33/58
CPCG01N33/56966G01N33/577G01N33/582G01N2800/22G01N2800/24
Inventor 李萡张玉平李扬秋黄桂璇郁志王顺清陈小惠周铭黎宇苗
Owner JINAN UNIVERSITY
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