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Immunoturbidimetry kit for determining procalcitonin in whole blood

A blood calcitonin and immune turbidimetric technology, which is applied in the field of medical testing, can solve the problems of low detection efficiency, inability to detect procalcitonin in whole blood samples, and high equipment cost, and achieves improved efficiency, significant anti-interference effect, and reduced The effect of equipment cost

Pending Publication Date: 2020-10-30
SUZHOU DIAGVITA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems in the prior art described in the background art that procalcitonin cannot be directly detected on whole blood samples, and serum or plasma needs

Method used

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  • Immunoturbidimetry kit for determining procalcitonin in whole blood
  • Immunoturbidimetry kit for determining procalcitonin in whole blood
  • Immunoturbidimetry kit for determining procalcitonin in whole blood

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Embodiment 1

[0016] An immunoturbidimetric method kit for measuring whole blood procalcitonin, which includes R1 reagent, R2 reagent and procalcitonin calibrator.

[0017] The buffer in the R1 reagent is MES buffer, the surfactant is brij35, the stabilizer is bovine serum albumin (BSA), the preservative is methylchloroisothiazolinone, and the sensitizer is polyethylene glycol 6000.

[0018] The latex microsphere particles labeled with procalcitonin antibody in the R2 reagent are, the buffer is MES buffer, and the preservative is methylchloroisothiazolinone.

[0019] In the original calcitonin calibration product, the procalcitonin protein is traced high-concentration procalcitonin protein, the preservative is methylchloroisothiazolinone, the buffer is HEPES buffer, and the stabilizer is bovine serum BSA.

[0020] 1. Preparation of reagent R1:

[0021] 1. Add 500mM MES buffer solution with pH=5.2 to deionized water and make it to a concentration of 50mM;

[0022] 2. Take 1L of the solutio...

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Abstract

The invention discloses an immunoturbidimetry kit for determining procalcitonin in whole blood. The kit comprises a reagent R1, a reagent R2 and a procalcitonin calibrator, the reagent R1 comprises abuffer solution, a surfactant, a stabilizer, a sensitizer and a preservative, the reagent R2 comprises latex microsphere particles marked with procalcitonin antibodies, a buffer solution, a preservative and a stabilizer, and the procalcitonin calibrator comprises procalcitonin protein, a preservative, a buffer solution and a stabilizer. According to the immunoturbidimetry kit for determining procalcitonin in whole blood, a whole blood sample can be directly added into the kit for quantitative detection of procalcitonin; the whole blood sample does not need to be centrifuged and serum or plasmadoes not need to be separated, the equipment cost can be reduced, the detection efficiency can be improved, the reagent has a remarkable anti-interference effect on red blood cells in the whole bloodsample and is high in detection precision, and the detection value deviation is smaller than 10% compared with that of a scheme adopting serum or plasma for detection.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an immunoturbidimetric methodological kit for measuring whole blood procalcitonin. Background technique [0002] Procalcitonin (PCT) is a prohormone composed of 116 amino acids with a molecular weight of approximately 12.7 kDa. Healthy human blood contains only a small amount of PCT. Its level in plasma increases in severe bacterial, fungal, parasitic infections, sepsis and multiple organ failure, PCT does not increase in autoimmune, allergic and viral infections, local limited bacterial infections, mild Infection and chronic inflammation do not cause it to rise. PCT reflects the active degree of systemic inflammatory response, and the increase of PCT level appears in severe shock, systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS). PCT (procalcitonin) is a highly specific and sensitive detection index for the diagnosis and dy...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/543
CPCG01N33/74G01N33/54313G01N2333/585
Inventor 贾引军金君玉赵年福张辉王明伟吴一凡
Owner SUZHOU DIAGVITA BIOTECH
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