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Kit for determining free thyroxine

A free thyroxine and kit technology, applied in the field of kits for the determination of free thyroxine, to achieve the effects of appropriate luminescence value, reduced dilution ratio, and high sensitivity

Pending Publication Date: 2020-11-06
SICHUAN ORIENTER BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a test kit for measuring free thyroxine, which solves the problem of the anti-interference ability of free thyroxine determination kits

Method used

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  • Kit for determining free thyroxine
  • Kit for determining free thyroxine
  • Kit for determining free thyroxine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A kit for measuring free thyroxine, comprising goat monoclonal antibody-coated magnetic beads, analysis buffer and enzyme working solution, the goat monoclonal antibody-coated magnetic beads are coated with biotin antibodies, the The biotin antibody is mouse IgG, the coating amount of the goat monoclonal antibody on the magnetic beads coated with the goat monoclonal antibody is 0.2 μg / mg, and the coating amount of the biotin antibody is 0.5 μg / mg.

[0033] In this embodiment, the preparation method of the goat monoclonal antibody-coated magnetic beads is as follows:

[0034] A. Take 0.25mg goat monoclonal antibody, and dialyze overnight at 2~8℃ with 5L of 0.02M PBS (PH7.4) buffer solution;

[0035] B. Take out the dialyzed goat monoclonal antibody in an appropriate container, accurately measure and record the volume, and dilute the goat monoclonal antibody to 0.5 mg / mL for use.

[0036] C. Weigh 1.0 mg of BNHS, fully dissolve it with 217 μL DMSO, and prepare a 10 mmol / ...

Embodiment 2

[0050] This example is based on Example 1, and the difference from Example 1 is that the biotin antibody is mouse IgG and rabbit IgG.

Embodiment 3

[0052] This example is based on Example 1, and the difference from Example 1 is that the biotin antibody is mouse IgG, rabbit IgG and bovine IgG.

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Abstract

The invention discloses a kit for determining free thyroxine, which comprises a sheep monoclonal antibody coated magnetic bead, an analysis buffer solution and an enzyme working solution, and the sheep monoclonal antibody coated magnetic bead is coated with a biotin antibody. According to the invention, the magnetic beads coated with the goat monoclonal antibody are coated with biotin antibodies.The magnetic beads coated with the goat monoclonal antibodies are coated with other antibodies, so that the stability of the kit can be improved, and the anti-interference capability of the kit duringdetection of clinical samples is further improved.

Description

technical field [0001] The invention relates to the technical field of free thyroxine detection, in particular to a kit for measuring free thyroxine. Background technique [0002] Thyroxine (T4) is the main thyroxine synthesized and secreted by thyroid follicular epithelial cells, generally as a precursor substance or prohormone. Most of the T4 secreted into the blood is combined with various proteins, and only 0.03% is biologically active and exists in the blood circulation in a free state (FT4). The determination of free thyroxine is not affected by serum binding proteins (mainly TBG), and is a sensitive indicator of thyroid function in vitro tests. Even when the concentration and binding force of serum thyroxine-binding globulin change under physiological and pathological conditions, it can more accurately reflect the function of the thyroid gland. [0003] Therefore, the determination of FT4 in blood can more truthfully evaluate the status of thyroid synthesis and secr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/78G01N33/577G01N33/543
CPCG01N33/78G01N33/577G01N33/54326
Inventor 李佳璐李玲
Owner SICHUAN ORIENTER BIOLOGICAL TECH
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