Cloning method of kosteletzkya virginica KvNAC gene and construction method of expression vector of kosteletzkya virginica KvNAC gene

A cloning method and gene technology, which are applied in the field of KvNAC gene cloning and expression vector construction of seaside mallow, can solve the problems of little research on the molecular mechanism of salt resistance and no KvNAC gene found.

Inactive Publication Date: 2020-11-10
LUDONG UNIVERSITY
2 Cites 0 Cited by

AI-Extracted Technical Summary

Problems solved by technology

Seaside mallow is a new salt-tolerant species introduced from abroad. At present, there are few studies on th...
View more

Abstract

The invention discloses a cloning method of a kosteletzkya virginica KvNAC gene and a construction method of an expression vector of the kosteletzkya virginica KvNAC gene. The cloning method comprisesthe steps of extracting total RNA of kosteletzkya virginica, performing cloning and sequence analyzing of the kosteletzkya virginica KvNAC gene, carrying out an RT-PCR reaction, and performing sequencing to obtain a full-length gene segment of the KvNAC gene; according to the obtained full length of the KvNAC gene, designing a primer for separation to obtain an ORF sequence of the gene; selectinghigh-fidelity Pfu DNA polymerase for PCR amplification to obtain a target segment; and taking 4 [mu]L of a PCR product and 1 [mu]L of a pEASY-Blunt Cloning Vector to carry out a ligation reaction soas to construct a recombinant cloning vector. The method is simple, convenient and effective; Isolation and cloning of the gene are realized in a new salt-resistant species, total RNA is successfullyextracted from the gene, and a spatial expression mode of the gene in a plant body is realized. The construction of the expression vector lays a foundation for the research of gene functions.

Application Domain

Microbiological testing/measurementFermentation +4

Technology Topic

Cloning vectorSequence analysis +8

Image

  • Cloning method of kosteletzkya virginica KvNAC gene and construction method of expression vector of kosteletzkya virginica KvNAC gene
  • Cloning method of kosteletzkya virginica KvNAC gene and construction method of expression vector of kosteletzkya virginica KvNAC gene
  • Cloning method of kosteletzkya virginica KvNAC gene and construction method of expression vector of kosteletzkya virginica KvNAC gene

Examples

  • Experimental program(1)
  • Effect test(1)

Example Embodiment

[0044] A method for cloning KvNAC gene of seaside mallow, including the following steps:
[0045] (1) Extraction of total RNA from seaside mallow:

PUM

PropertyMeasurementUnit
Pre-denatured94.0°C
Molecular weight28.26

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products