Leukocyte classification counting method and device and blood analyzer

Abstract

The invention provides a leukocyte classification counting method and device and a blood analyzer, and the method comprises the steps: obtaining an initial leukocyte histogram of leukocytes in a bloodsample, wherein the initial leukocyte histogram is a quantity and volume distribution histogram of leukocytes; defining a blood shadow area in the initial leukocyte histogram; and correcting the blood shadow region to obtain a corrected lymphocyte region. According to the leukocyte counting and classifying method, the leukocyte counting and classifying device and the blood analyzer provided by the embodiment of the invention, the interference of the blood shadow area to the lymphocyte area in the leukocyte histogram can be weakened and even eliminated, and the leukocyte counting and classifying accuracy is improved.

Description

technical field [0001] The present invention generally relates to the technical field of blood analysis, and more specifically relates to a method, a device and a blood analyzer for differential counting of white blood cells. Background technique [0002] Impedance method is one of the classic methods for counting and classifying white blood cells in human body, and it is also widely used in counting and classifying white blood cells in animals. The white blood cell count and classification of animals play an important role in the diagnosis and treatment of animals. On the other hand, due to certain differences in the morphology of human blood cells and animal blood cells, in the actual detection process, the reaction conditions, reaction temperature, and reagent dosage are not completely the same; especially the amount of hemolytic agent used has a significant effect on the detection of animal blood cells. Impact. For example: the same amount and concentration of hemolyti...

AI Technical Summary

Problems solved by technology

On the other hand, due to certain differences in the morphology of human blood cells and animal blood cells, in the actual detection process, the reaction conditions, reaction temperature, and reagent dosage are not completely the same; especially the amount of hemolytic agent used has a significant effect on the detection of animal blood cells. Impact

For example: the same amount and concentration of hemolytic agents can destroy human red blood cells, and the shape of the white blood cell histogram of human blood is better, which can fully meet the requirements of counting and classification. However, it cannot destroy the red blood cells of some animals, resulting in lymphatic There are a large number of red blood cell fragments in the cell area, which interferes with the classification and counting of animal white blood cells; if the amount of hemolytic agent is increased, the damage to animal white blood cells will be more obvious, resulting in more overlapping volume distribution of each particle group in the white blood cell histogram, which will cause serious classification results. not allowed

Therefore, in the current detection of white blood cells in animals, the interference of cell debris inevitably exists in the lymphatic region of the white blood cell histogram, which affects the counting and classification of white blood cells; this is the current challenge of the impedance method in the detection of white blood cells in animals

Images