Two-stage froth flotation method for concentrating and separating protein in purple perilla cakes

A technology of froth flotation and perilla cake, which is applied in the fields of peptide preparation, chemical instruments and methods, organic chemistry, etc., can solve the problems of easily polluted environment, complicated process, poor concentration effect, etc.

Active Publication Date: 2020-12-01
ZHONGBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention overcomes the shortcomings of other protein separation technologies such as poor concentration effect, high equipment cost, complicated process and easy pollution of the environment.

Method used

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  • Two-stage froth flotation method for concentrating and separating protein in purple perilla cakes
  • Two-stage froth flotation method for concentrating and separating protein in purple perilla cakes
  • Two-stage froth flotation method for concentrating and separating protein in purple perilla cakes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1: the first stage froth flotation tower

[0067] A froth flotation tower (such as figure 2 , 3 As shown), it comprises a hollow cylindrical foam flotation column 1 with a diameter of 50mm and a height of 1.3m, and an hourglass-shaped member 2 is installed in the foam flotation column 1, and the hourglass-shaped member 2 consists of two equivalent Hollow, bottomless round platform top edge butt joint structure, bottom edge seamlessly connected with the inner wall of the foam flotation column 11, the top diameter of the round platform is 1 / 2 of the bottom surface diameter, the bottom surface diameter is 50mm, and the hourglass-shaped member 2 The vertical height is 225mm, and the junction of the two circular frustums is located in the middle of the foam phase.

[0068] Among them, the material of the hourglass-shaped component is a plastic sheet with a thickness of 1mm. The sheet is made into a hollow round platform through a conventional thermoplastic proc...

Embodiment 2

[0069] Embodiment 2: the first stage froth flotation tower

[0070] A froth flotation tower (such as figure 2 , 3 As shown), it comprises a hollow cylindrical foam flotation column 1 with a diameter of 50mm and a height of 1.3m, and an hourglass-shaped member 2 is installed in the foam flotation column 1, and the hourglass-shaped member 2 consists of two equivalent Hollow, bottomless round platform top edge butt joint structure, bottom edge seamlessly connected with the inner wall of the foam flotation column 11, the top diameter of the round platform is 1 / 2 of the bottom surface diameter, the bottom surface diameter is 50mm, and the hourglass-shaped member 2 The vertical height is 200mm, and the junction of the two circular frustums is located in the middle of the foam phase.

[0071] Among them, the material of the hourglass-shaped component is a plastic sheet with a thickness of 1mm. The sheet is made into a hollow round platform through a conventional thermoplastic proc...

Embodiment 3

[0072] Embodiment 3: the first stage froth flotation tower

[0073] A froth flotation tower (such as figure 2 , 3 As shown), it comprises a hollow cylindrical foam flotation column 1 with a diameter of 50mm and a height of 1.3m, and an hourglass-shaped member 2 is installed in the foam flotation column 1, and the hourglass-shaped member 2 consists of two equivalent Hollow, bottomless round platform top edge butt joint structure, bottom edge seamlessly connected with the inner wall of the foam flotation column 11, the top diameter of the round platform is 1 / 2 of the bottom surface diameter, the bottom surface diameter is 50mm, and the hourglass-shaped member 2 The vertical height is 250mm, and the junction of the two circular frustums is located in the middle of the foam phase.

[0074] Among them, the material of the hourglass-shaped component is a plastic sheet with a thickness of 1mm. The sheet is made into a hollow round platform through a conventional thermoplastic proc...

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Abstract

The invention provides a method for concentrating and separating protein in purple perilla cake meal through a two-stage froth flotation method. The method comprises the following steps that 1, purpleperilla cake meal leaching liquor is prepared; 2, in the first-stage froth flotation, a froth flotation tower provided with a hourglass-shaped component is used for strengthening froth liquid drainage, the enrichment ratio of purple perilla protein is increased, and an obtained concentrated solution can be directly used as a raw material for producing purple perilla protein powder; and 3, the first-stage residual liquid is used as the feed liquid in the second-stage froth flotation, so that the recovery effect of the purple perilla protein is further improved. The method has the advantages ofgreen process, low energy consumption, low investment, simple steps and the like, effectively solves the problem of concentration and separation of the purple perilla protein, and has good economic and social benefits.

Description

technical field [0001] The invention relates to the field of recycling and concentrating waste protein in chemical industry separation process, in particular to a method for concentrating and separating protein in perilla cake by two-stage foam flotation. Background technique [0002] Basil (Perilla frutescens L.) is one of the first batch of medicinal and edible plants promulgated by the Ministry of Health, and plays an important role in the construction of the general health industry and functional agriculture. The by-product of perilla seed oil extraction and degreasing—the protein content in perilla cake is high (28-45%), and the essential amino acid composition mode meets the standards stipulated by FAO (Food and Agriculture Organization of the United Nations) and WHO (World Health Organization). Proteins such as rapeseed meal and cottonseed meal contain toxic components such as glucosinolates and gossypol. They are high-quality plant protein resources and can be widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 胡楠张志军李会珍侯天宇张可可
Owner ZHONGBEI UNIV
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