Method for generating cells of t cell lineage

Abstract

A method of generating cells of a T cell lineage is provided comprising (a) culturing a sample comprising stem cells or progenitor cells with a Notch ligand conjugated to a suspension support and (b)isolating cells of the T cell lineage. In one embodiment, the cells of the T-cell lineage are progenitor T cells or mature T cells. Compositions, kits and uses thereof are also provided.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to U.S. Provisional Application No. 62 / 630,497, filed February 14, 2018, the contents of which are incorporated herein by reference in their entirety. technical field [0003] The present application relates to methods, compositions and kits for generating cells of T cell lineage and uses thereof. In particular, the present application relates to methods, compositions and kits for generating progenitor cells and mature T cells and uses thereof. Background technique [0004] T cells are key mediators of adaptive immunity and can be used as therapeutic agents in immunotherapy against pathogens and cancer. Hematopoietic stem cell transplantation (HSCT) provides an effective treatment for a wide range of malignant and non-malignant conditions, but the conditioning regimens required prior to treatment result in delayed T cell recovery (Krenger et al., 2011). In contrast to m...

AI Technical Summary

Problems solved by technology

While this progress is encouraging, the use of these methods to generate proT cells for therapeutic use is limited by the need for scale-up in clinical production and is not routinely produced in clinically usable large-scale cell numbers

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