Single-cell mass spectrometry system and method

A mass spectrometry and single-cell technology, applied in the field of cell analysis, can solve problems such as inaccurate positioning, cross-contamination, and cumbersome steps, and achieve the effects of improving judgment accuracy, improving efficiency and precision, and high analysis accuracy

Pending Publication Date: 2020-12-11
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, a problem in the field of single-cell detection is that there is no uniform standard for sufficient consideration of cell extraction, which is simply obtained by researchers through limited experiments, without considering the specificity of cells, and the results obtained are inaccurate. cumbersome
[0005] 1. The U-shaped probe does not move, and the operating platform moves: resultin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1:

[0037] figure 1 A structural diagram of a single-cell mass spectrometry system according to Example 1 of the present invention is shown schematically, such as figure 1 As shown, the single cell mass spectrometry system includes:

[0038] The carrier 11, and the plurality of cells are adapted to be carried by the carrier and separated from each other;

[0039] The extraction unit 21, the extracting unit comprises an extraction pipe and a discharge electrode, and an extract in the conduit forms a spray exiting from the bottom of the pipe under the action of the discharge electrode;

[0040] Moving units such as a three-dimensional mobile platform, the pipe or carrier is driven under the moving unit such that the bottom end of the extraction liquid conduit is selectively moved to the upper side of any cell;

[0041] The isolation unit 41, such as a spacer cover, the isolation unit for forming a space that isolates any cell from other cells; the bottom end of the ...

Example Embodiment

[0066] Example 2:

[0067] Application examples of single cell mass spectrometry system and method according to Embodiment 1 of the present invention.

[0068] In this application example, the carrier is a matrix structure, having a rectangular groove that is array-distributed, each groove adapted to accommodate a single cell;

[0069] The isolation unit is separated from the isolation cover, from the upper and lower isolation hoods, the inside of the spacer is large, the bottom end of the spacer is a rectangular shape that matches the groove of the carrier; the isolation cover is over the carrier On the groove, a space that is isolated from other grooves is formed, and there is only one rectangular groove in the space, i.e., only one cell in the space;

[0070] The mobile unit uses a three-dimensional mobile platform for driving the carrier three-dimensional movement, such as moving movement in the horizontal plane, so that the isolation cover (relatively) moves to any of the rec...

Example Embodiment

[0086] Example 3:

[0087] According to an application example of a single cell mass spectrometry system and method according to Embodiment 1 of the present invention, it is:

[0088] The carrier fixation, the mass spectrometer is fixed, the isolation cover, the extraction unit and the collecting unit are relatively fixed, and the three-dimensional moving spacer is driven to the same moving unit, thereby deduction on a rectangular groove having a cell at the designated position; While forming a spaced space on the rectangular groove, the bottom end of the extract is in the upper side of a single cell in the space.

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Abstract

The invention provides a single-cell mass spectrometry system and method. The system comprises a bearing part, an extraction unit, a moving unit, an isolation unit, a collecting unit, a detection unit; a plurality of cells are suitable for being borne by the bearing part and are separated from each other; the extraction unit comprises an extraction liquid pipeline and a discharge electrode; an extraction liquid in the pipeline forms spray emitted from an opening in the bottom end of the pipeline under the action of the discharge electrode; under the driving of the moving unit, the pipeline orthe bearing piece enables the bottom end of the extraction liquid pipeline to selectively move to the upper side of any cell; the isolation unit is used for forming a space for isolating any cell fromthe other cells; the bottom end of the extraction liquid pipeline is suitable for being located in the space; the collecting unit is used for collecting substances obtained after the cells in the space are extracted; and the detection unit is used for detecting whether the cells in the space are fully extracted or not. The system has the advantages of high precision, high efficiency and the like.

Description

technical field [0001] The invention relates to cell analysis, in particular to a single cell mass spectrometry analysis system and method. Background technique [0002] Cells are the basic units that make up living organisms. To understand the changes and behaviors of individual cells at various stages in a complex and changeable environment, methods for single-cell analysis are required. Mass spectrometry is a method for simultaneous analysis of multiple components. According to the different molecular weights of various components in cells, spectral peaks arranged according to mass numbers can be formed in mass spectrometry, and further through multi-stage mass spectrometry analysis, various components in cells can be obtained. molecular information. Since mass spectrometry does not require labels and does not need to know the information of the molecules to be tested in advance, it can quickly identify various unknown components in cells and obtain the omics information...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N15/10
CPCG01N15/1031G01N15/1404G01N2015/1006G01N2015/0065Y02A50/30
Inventor 闻路红刘云陈腊陈安琪胡舜迪洪欢欢李刚强
Owner NINGBO UNIV
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