Single-cell mass spectrometry system and method

Abstract

The invention provides a single-cell mass spectrometry system and method. The system comprises a bearing part, an extraction unit, a moving unit, an isolation unit, a collecting unit, a detection unit; a plurality of cells are suitable for being borne by the bearing part and are separated from each other; the extraction unit comprises an extraction liquid pipeline and a discharge electrode; an extraction liquid in the pipeline forms spray emitted from an opening in the bottom end of the pipeline under the action of the discharge electrode; under the driving of the moving unit, the pipeline orthe bearing piece enables the bottom end of the extraction liquid pipeline to selectively move to the upper side of any cell; the isolation unit is used for forming a space for isolating any cell fromthe other cells; the bottom end of the extraction liquid pipeline is suitable for being located in the space; the collecting unit is used for collecting substances obtained after the cells in the space are extracted; and the detection unit is used for detecting whether the cells in the space are fully extracted or not. The system has the advantages of high precision, high efficiency and the like.

Description

technical field [0001] The invention relates to cell analysis, in particular to a single cell mass spectrometry analysis system and method. Background technique [0002] Cells are the basic units that make up living organisms. To understand the changes and behaviors of individual cells at various stages in a complex and changeable environment, methods for single-cell analysis are required. Mass spectrometry is a method for simultaneous analysis of multiple components. According to the different molecular weights of various components in cells, spectral peaks arranged according to mass numbers can be formed in mass spectrometry, and further through multi-stage mass spectrometry analysis, various components in cells can be obtained. molecular information. Since mass spectrometry does not require labels and does not need to know the information of the molecules to be tested in advance, it can quickly identify various unknown components in cells and obtain the omics information...

AI Technical Summary

Problems solved by technology

[0003] At present, a problem in the field of single-cell detection is that there is no uniform standard for sufficient consideration of cell extraction, which is simply obtained by researchers through limited experiments, without considering the specificity of cells, and the results obtained are inaccurate. cumbersome

[0005] 1. The U-shaped probe does not move, and the operating platform moves: resulting in micro-displacement of cells, inaccurate positioning, and cross-contamination

[0006] 2. The U-shaped recess partially blocks the field of view of the microscope: the operator cannot use the microscope camera to locate the cell position, and can only remove the U-shaped probe after extracting a cell, and then operate after observing the next position, which is cumbersome and difficult to locate. allow

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