Plasma anaerobic microorganism breeding device and method thereof
An anaerobic microorganism and plasma technology, which is applied in the field of plasma anaerobic microorganism breeding equipment, can solve the problems that it is difficult to reach the anaerobic environment level of anaerobic industrial bacteria, and the anaerobic environment is harsh, so as to achieve a beneficial breeding effect and relatively Possibility of hypoxic poisoning and the effect of simple experimental operation
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[0073] Embodiment The plasma anaerobic microorganism breeding device of the present invention and the conventional plasma mutagenesis breeding device treat anaerobic microorganism mutagenesis
[0074] 1. Experimental strain: Clostridium butyricum
[0075] 2. Composition and formula of strain culture medium: yeast extract 3g, beef extract 10g, tryptone 10g, glucose 5g, soluble starch 1g, sodium chloride 5g, sodium acetate trihydrate 3g, cysteine hydrochloride 0.15 g. Agar 15g (for solid medium), distilled water 1 000mL; adjust pH to 7.4; sterilize at 121°C for 20min.
[0076]3. Equipment: the device of the present invention, ARTP atmospheric pressure room temperature plasma mutagenesis breeding instrument (ARTP-IIS), petri dish
[0077] 4. Experimental mutagenesis conditions: the device of the present invention and the ARTP atmospheric room temperature plasma mutagenesis breeding instrument (ARTP-IIS) all adopt: power 120W, gas volume 10SLM, treatment distance 2mm, cooling w...
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