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Plasma anaerobic microorganism breeding device and method thereof

An anaerobic microorganism and plasma technology, which is applied in the field of plasma anaerobic microorganism breeding equipment, can solve the problems that it is difficult to reach the anaerobic environment level of anaerobic industrial bacteria, and the anaerobic environment is harsh, so as to achieve a beneficial breeding effect and relatively Possibility of hypoxic poisoning and the effect of simple experimental operation

Pending Publication Date: 2020-12-15
LUOYANG TMAXTREE BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This patent uses nitrogen filling to achieve anaerobic environment. Although this method meets certain anaerobic conditions, in the application of industrial bacteria, the anaerobic environment is very harsh, and it is difficult to achieve the anaerobic environment level that anaerobic industrial bacteria need to achieve.

Method used

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  • Plasma anaerobic microorganism breeding device and method thereof
  • Plasma anaerobic microorganism breeding device and method thereof
  • Plasma anaerobic microorganism breeding device and method thereof

Examples

Experimental program
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Effect test

Embodiment

[0073] Embodiment The plasma anaerobic microorganism breeding device of the present invention and the conventional plasma mutagenesis breeding device treat anaerobic microorganism mutagenesis

[0074] 1. Experimental strain: Clostridium butyricum

[0075] 2. Composition and formula of strain culture medium: yeast extract 3g, beef extract 10g, tryptone 10g, glucose 5g, soluble starch 1g, sodium chloride 5g, sodium acetate trihydrate 3g, cysteine ​​hydrochloride 0.15 g. Agar 15g (for solid medium), distilled water 1 000mL; adjust pH to 7.4; sterilize at 121°C for 20min.

[0076]3. Equipment: the device of the present invention, ARTP atmospheric pressure room temperature plasma mutagenesis breeding instrument (ARTP-IIS), petri dish

[0077] 4. Experimental mutagenesis conditions: the device of the present invention and the ARTP atmospheric room temperature plasma mutagenesis breeding instrument (ARTP-IIS) all adopt: power 120W, gas volume 10SLM, treatment distance 2mm, cooling w...

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Abstract

The invention relates to a plasma anaerobic microorganism breeding device. The plasma anaerobic microorganism breeding device comprises an anaerobic microorganism operation processing module, a detection module and a control module. The anaerobic microorganism operation processing module is composed of an operation chamber and a sampling chamber connected with the operation chamber through a transfer door. According to the invention, mutagenesis treatment and a continuous culture function of anaerobic bacteria at less than 0.03% of oxygen content are realized.

Description

technical field [0001] The invention belongs to the field of plasma technology, and in particular relates to a plasma anaerobic microorganism breeding device and a method thereof. Background technique [0002] Anaerobic microorganisms widely exist in nature, and they are inseparable from the environment that human beings depend on for survival and living. However, in the presence of oxygen, anaerobic microorganisms inhibit their growth and even play a toxic role, so the breeding of anaerobic microorganisms has been restricted. limit. How to use efficient microbial mutagenesis technology to achieve rapid optimization of strains is an important task in microbial breeding. [0003] Plasma is the fourth state of matter that coexists with the solid, liquid, and gas states of matter. It is a state of matter that is mainly composed of a large number of free electrons and charged ions, and is electrically neutral macroscopically. Studies have shown that a large number of active pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42C12M1/38C12M1/36C12M1/34C12M1/04C12N13/00
CPCC12M29/06C12M35/00C12M41/12C12M41/34C12M41/44C12M41/48C12N13/00
Inventor 王立言段保峰周震
Owner LUOYANG TMAXTREE BIOTECH CO LTD
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