Cyathane diterpene compound and application thereof
The technology of a compound, ornithane, applied in the field of medicine, can solve problems such as short half-life, unsatisfactory pharmacokinetics, and large relative molecular weight, and achieve the effect of promoting synaptic growth and significant neurotrophic activity
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Embodiment 1
[0034] preparation of
[0035] 1. Liquid fermentation culture
[0036] Saccharomyces cerevisiae LSc-1 was transferred to liquid YPD medium, and cultured at 30° C. and 200 rpm for 2 days to obtain a seed culture medium. The seed solution was inoculated into the YPD medium at a ratio of 1:1000 (each 1000mL Erlenmeyer flask was filled with 500mL YPD medium, and 10 Erlenmeyer flasks were set). Culture at 30° C. and 200 rpm for 5 days to obtain a fermentation broth.
[0037] YPD medium (natural pH): Peptone 20g, Yeast Extract 10g, glucose 20g, distilled water to 1L.
[0038] 2. Preparation of compounds
[0039] Take the fermentation broth obtained in step 1, add 500 ml of ethyl acetate to each Erlenmeyer flask for leaching at room temperature (20°C) for 24 hours (perform ultrasonic treatment every 12 hours during this period, and each ultrasonic treatment is 50 Hz continuous ultrasonic for 20 min) , take the supernatant; add 500 milliliters of new ethyl acetate to the remaini...
Embodiment 2
[0052] preparation of
[0053] 1. Liquid fermentation culture
[0054] Saccharomyces cerevisiae LSc-2 was transferred to liquid YPD medium, and cultured at 30° C. and 200 rpm for 2 days to obtain a seed culture solution. The seed solution was inoculated into the YPD medium at a ratio of 1:1000 (each 1000mL Erlenmeyer flask was filled with 500mL YPD medium, and 10 Erlenmeyer flasks were set). Culture at 30° C. and 200 rpm for 5 days to obtain a fermentation broth.
[0055] YPD medium (natural pH): Peptone 20g, Yeast Extract 10g, glucose 20g, distilled water to 1L.
[0056] 2. Preparation of compounds
[0057] Take the fermentation broth obtained in step 1, add 500 ml of ethyl acetate to each Erlenmeyer flask for leaching at room temperature (20°C) for 24 hours (perform ultrasonic treatment every 12 hours during this period, and each ultrasonic treatment is 50 Hz continuous ultrasonic for 20 min) , take the supernatant; add 500 milliliters of new ethyl acetate to the remai...
Embodiment 3
[0069] preparation of
[0070] 1. Liquid fermentation culture
[0071] Saccharomyces cerevisiae LSc-3 was transferred to liquid YPD medium, and cultured at 30° C. and 200 rpm for 2 days to obtain a seed culture medium. The seed solution was inoculated into the YPD medium at a ratio of 1:1000 (each 1000mL Erlenmeyer flask was filled with 500mL YPD medium, and 10 Erlenmeyer flasks were set). Culture at 30° C. and 200 rpm for 5 days to obtain a fermentation broth.
[0072] YPD medium (natural pH): Peptone 20g, Yeast Extract 10g, glucose 20g, distilled water to 1L.
[0073] 2. Preparation of compounds
[0074] Take the fermentation broth obtained in step 1, add 500 ml of ethyl acetate to each Erlenmeyer flask for leaching at room temperature (20°C) for 24 hours (perform ultrasonic treatment every 12 hours during this period, and each ultrasonic treatment is 50 Hz continuous ultrasonic for 20 min) , take the supernatant; add 500 milliliters of new ethyl acetate to the remaini...
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