Kumquat essential oil and preparation method and application thereof

A technology of kumquat essential oil and kumquat essence, which is applied in the field of traditional Chinese medicine, can solve problems such as non-negligible, target organ damage and side effects, and achieve the effects of reducing target organ damage, reducing vasoconstrictive pressure, and easy availability of resources.

Active Publication Date: 2020-12-22
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the treatment of hypertension is a long-term process, and the side effects of target organ damage cannot be ignored.

Method used

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  • Kumquat essential oil and preparation method and application thereof
  • Kumquat essential oil and preparation method and application thereof
  • Kumquat essential oil and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of kumquat essential oil

[0029] 1. Kumquat raw material: purchased from the Suichuan planting base in Jiangxi Province, identified and reported by Mr. Liu Yong, School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, as the fruit of Fortunella Crassifolia Swingle, a plant of the genus Kumquat in the family Rutaceae.

[0030] 2. Preparation method 1) Pre-treatment: After cleaning the fresh kumquat, crush it;

[0031] 2) Dry steam extraction: Weigh 12 kg of kumquat crushed in step 1 and carry out dry steam extraction in the essential oil extraction system device. The extraction time is 2 hours, stop the instrument, and after the steam condenses, use a 10ml syringe to extract the layered liquid. Collect the essential oil layer to obtain crude kumquat essential oil;

[0032] 3) Refining: put the crude kumquat essential oil prepared in step 2 into a brown bottle, seal the bottle cap with a parafilm, refrigerate and let stand overnight at 4°C in...

Embodiment 2

[0034] Determination of Active Components in Kumquat Essential Oil

[0035] 1. Experimental process

[0036] Gas chromatography conditions: Agilent 19091s-433:93.92873 column, 30m×0.25mm×0.25μm. The inlet temperature was 250 °C, the total flow rate was 19.6 mL / min, the split ratio was 20:1, and the column flow rate was 1 mL / min. The interface temperature is 280°C. The heating program is 40°C for 2 minutes, rising to 250°C at 10°C / min, holding for 2 minutes, then rising to 280°C at 20°C / min, and holding for 1 minute. The carrier gas is high purity helium. The injection volume was 1 μL.

[0037] Mass spectrometry conditions: EI ionization source, electron energy at 70eV, ion source temperature at 230°C, quadrupole temperature at 150°C. The measurement method is full scan, the mass range is 35-550u, the multiplier voltage is 1482V, and the filament emission current is 34.6μA. The solvent delay time was 2.0 min.

[0038] Qualitative and quantitative: Use the NIST11.L spectr...

Embodiment 3

[0042] Animal Experiments on Prevention and Treatment of Hypotension

[0043] A single dose of kumquat essential oil 0.5ml / kg was administered, and the systolic blood pressure, serum angiotensin Ⅱ, aldosterone, and vascular endothelial relaxation factor were investigated in rats.

[0044] 1. Experimental protocol

[0045] 1.1 Drug preparation

[0046] Get the kumquat essential oil prepared in Example 1 and dilute it with 2% Tween 80 aqueous solution.

[0047] 1.2 Animals

[0048] SPF grade SD rats, male, weighing (180±10) g, 30 were purchased from the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine [SCXK (Gan) 2018-0003].

[0049] Raised in the experimental animal barrier system [SYXK (Gan) 2017-0004], the laboratory temperature is 23±2°C, the relative humidity is 53±3%, the noise is below 60 decibels, the sunshine is 12h, the animals are free to drink water and eat, and the bedding is changed every day , when the body weight of the animals...

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Abstract

The invention discloses kumquat essential oil and a preparation method and application thereof. The kumquat essential oil is a pure natural substance extracted from kumquat fruits. The kumquat essential oil is obtained through dry steaming extraction, main active ingredients and content in the kumquat essential oil are measured by utilizing GC-MS, and then a hypertension animal model test is established, so that the kumquat essential oil and the active ingredients thereof are proved to be capable of obviously reducing vasoconstriction pressure and regulating vasodilatation factors such as angiotensin II and the like and also capable of relieving target organ damage caused by hypertension; and a cell test proves that the kumquat essential oil is capable of regulating the angiotensin II to induce HUVEC cell viability. Therefore, the kumquat essential oil has good prevention and treatment effects on renal hypertension.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a kumquat essential oil and its preparation method and application. Background technique [0002] With the continuous improvement of people's living standards and great changes in dietary structure, the incidence and death of cardiovascular and cerebrovascular diseases in my country are also increasing. Among the 35-75-year-old population, the prevalence and awareness of hypertension in Chinese adults The control rate and control rate were 37.2%, 22.9% and 5.7% respectively, and the antihypertensive compliance rate is still lower than that of western developed countries. In addition to improving patients' awareness of hypertension treatment, the research on hypertension drugs also needs urgent attention The problem. [0003] Kumquat, also known as Luofu (Oujiang Yizhi), longevity kumquat, milk orange (flower mirror), Gongsun orange, kumquat, etc., is the fru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/75A61P13/12A61P9/12
CPCA61K36/75A61K2236/13A61K2236/331A61P9/12A61P13/12
Inventor 曾治君刘红宁张吉日木吐吉燕华严小军
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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