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Goat pox virus proliferation method, goat pox live vaccine as well as preparation method and application of goat pox live vaccine

A technology of goat pox virus and goat pox, which is applied in the direction of viruses, vaccines, antiviral agents, etc., can solve the problems of unstable quality of primary cells, restrictions on the stable production of vaccines in batches, and difficulty in control, so as to alleviate the problem of low cost and unsustainable Stable, conducive to large-scale proliferation, and alleviate the effect of poor stability

Active Publication Date: 2020-12-25
天康制药股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The traditional preparation method of goat pox virus is to collect sheep testes, prepare sheep testis primary cells and culture them on the spinner bottle, but there are the following disadvantages, which restrict the stable batch production of the vaccine
[0005] 1. It is difficult to obtain sheep testicles. Sheep testicles need to be collected on sheep farms. The season is strong, and there is a risk of brucellosis among the collectors.
[0006] 2. The quality of primary cells is unstable, easily polluted, and difficult to control, resulting in large differences in vaccine quality between batches
[0007] 3. The spinner bottle culture process of adherent cells is used for production, which is cumbersome to operate, easy to pollute, and heavy workload, which has become the main obstacle to the large-scale production of the vaccine at present

Method used

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  • Goat pox virus proliferation method, goat pox live vaccine as well as preparation method and application of goat pox live vaccine
  • Goat pox virus proliferation method, goat pox live vaccine as well as preparation method and application of goat pox live vaccine
  • Goat pox virus proliferation method, goat pox live vaccine as well as preparation method and application of goat pox live vaccine

Examples

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Effect test

preparation example Construction

[0067] Goat pox is a highly contagious infectious disease that seriously harms goats caused by goat pox virus. At present, the most effective prevention method is to inoculate goat pox live vaccine, and the raw material of the vaccine is goat pox virus. The traditional preparation method of goat pox virus is to collect sheep testis, prepare sheep testis primary cells, carry out adherent culture of the primary cells on a spinner bottle, inoculate and culture the virus. However, this method has problems such as difficulty in obtaining sheep testis, unstable quality of primary cells, and cumbersome process, which restrict the stable batch production of the vaccine. In view of this, it is proposed that the sheep pox virus propagation method of the present invention comprises the following steps:

[0068] First, the goatpox virus is subcultured and domesticated on a suspension culture cell line to obtain the domesticated virus. Virus subculture and domestication method, that is, ...

Embodiment 1

[0136] Will grow well at a concentration of 4.0×10 6 Each / mL cell line was placed in a 500mL shake flask, and the virus was inoculated at a dose of 0.02MOI. The culture conditions were: temperature 36-38°C, 5% CO 2 , 60% relative humidity, 125rpm rotation speed. The virus cultured for 96 hours was taken as progeny, and subcultured and acclimated once in this way. During the last subculture and domestication, samples were taken at 48, 72, 96, and 120 hours after inoculation to detect the virus content. The results are shown in Table 1.

Embodiment 2

[0138] The difference from Example 1 is that 2 generations are passed down and domesticated.

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Abstract

The invention provides a goat pox virus proliferation method, a goat pox live vaccine and a preparation method and application of the goat pox live vaccine, and relates to the technical field of vaccine preparation. The goat pox virus proliferation method provided by the invention comprises the following steps of carrying out passage domestication on goat pox viruses on a suspension culture cell line to obtain domesticated viruses; carrying out suspension amplification culture on the cell line; and inoculating the domesticated viruses to the cell line subjected to the amplification culture, and performing culture to obtain proliferated viruses. According to the virus proliferation method, large-scale suspension culture of the cell line is realized, the adaptability of the viruses to cellsis improved, and the goat pox viruses with high and stable viral titer can be obtained. The preparation method of the goat pox virus live vaccine provided by the invention is simple in process and lowin cost, and the technical problems of virus damage, poor vaccine stability and short preservation time in a vaccine preparation process are relieved. The goat pox live vaccine provided by the invention is safe and effective, and can be applied to prevention and control of goat pox.

Description

technical field [0001] The invention relates to the technical field of vaccine preparation, in particular to a method for multiplying goat pox virus, a live goat pox vaccine and a preparation method and application thereof. Background technique [0002] Goat pox is a highly contagious infectious disease that seriously harms goats caused by goat pox virus. Goat pox is widely distributed in many countries in Asia, Europe, North America and Africa. It is currently prevalent in some countries in Africa, the Middle East and Asia, causing great harm to the sheep industry. The International Organization for Animal Health (OIE) listed the disease as It is a major infectious disease of category A, and it is also listed as a first-class animal disease in my country. Goat pox mainly occurred in 9 provinces including Inner Mongolia, Gansu, Ningxia, Xinjiang, Tibet, Zhejiang, and Hebei in the early days of the founding of the People's Republic of China. In the mid-1960s, comprehensive e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/275A61P31/20
CPCC12N7/00A61K39/12A61P31/20A61K2039/552A61K2039/5254C12N2710/24234C12N2710/24251
Inventor 贺笋潘晓梅王遵宝李俊辉李晓梅张慧敏侯红娟毛丽萍周涛郭晶
Owner 天康制药股份有限公司
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