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Automatic tracking and quality control method for iPSC and terminal equipment

A quality control method and automatic tracking technology, applied in the field of stem cell culture, can solve the problems of restricting large-scale iPSC culture and achieve the effect of solving automation problems

Pending Publication Date: 2020-12-25
SHENZHEN UNIV
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide an automatic tracking and quality control method and terminal equipment for iPSC in view of the deficiencies in the prior art, aiming to solve the problem that the tracking and quality control of existing iPSC clones are mainly done manually, This limits the problem of large-scale culture of iPSC

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  • Automatic tracking and quality control method for iPSC and terminal equipment
  • Automatic tracking and quality control method for iPSC and terminal equipment
  • Automatic tracking and quality control method for iPSC and terminal equipment

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Embodiment Construction

[0040] The present invention provides an automatic tracking and quality control method for iPSC and terminal equipment. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below with reference to the accompanying drawings and examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0041] Those skilled in the art will understand that unless otherwise stated, the singular forms "a", "an", "said" and "the" used herein may also include plural forms. It should be further understood that the word "comprising" used in the description of the present invention refers to the presence of said features, integers, steps, operations, elements and / or components, but does not exclude the presence or addition of one or more other features, Integers, steps, operations, elements...

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Abstract

The invention discloses an automatic tracking and quality control method for iPSC and terminal equipment, and the method comprises the steps of enhancing data of an original iPSC clone microscopic image to acquire an enhanced iPSC clone microscopic image; performing feature extraction on the enhanced iPSC clone microscopic image by adopting a feature pyramid network to obtain image feature information; performing feature fusion on the image feature information by adopting a weighted bidirectional feature pyramid network to obtain image fusion features; and convoluting the image fusion featuresthrough a detection head, and outputting a quality control result and positioning information of the iPSC clone. According to the method provided by the invention, real-time and visual tracking and quality monitoring of iPSC clone can be realized under the condition of ensuring relatively high classification accuracy, and important technical support is provided for large-scale production of iPSC.

Description

technical field [0001] The invention relates to the technical field of stem cell culture, in particular to an automatic tracking and quality control method and terminal equipment for iPSC. Background technique [0002] Induced pluripotent stem cells (iPSCs), like embryonic stem cells (ESCs), have the ability to differentiate into all organs and tissues of adults, and have the advantages of little ethical controversy and avoiding rejection. Therefore, iPSCs are widely used in regenerative medicine and tissue engineering. , Drug development, disease model construction and other fields have great application potential, which has impacted the status of ESC in regenerative medicine to a certain extent. However, at present, there are technical challenges when iPSC technology is put into practical application. One of them is that the tracking and quality control of iPSC clones during the reprogramming process are mainly done manually, which limits the large-scale cultivation of iPS...

Claims

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Application Information

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IPC IPC(8): G06K9/00G06K9/46G06K9/62
CPCG06V20/695G06V20/698G06V10/56G06F18/253
Inventor 汪天富王兴猎雷柏英岳广辉周光前廖进齐
Owner SHENZHEN UNIV
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