Unlock instant, AI-driven research and patent intelligence for your innovation.

Microscopic imaging device and microscopic imaging method for accelerating cell sedimentation

A microscopic imaging and cell technology, applied in measuring devices, chemical instruments and methods, particle and sedimentation analysis, etc., can solve the problems of cell height differences and long natural sedimentation time, and achieve the effect of improving quality

Pending Publication Date: 2021-01-01
南京九川科学技术有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cells of different sizes are finally stabilized at slightly different heights in the liquid, and it takes a long time for the cell suspension to settle naturally in the microfluidic chamber, and even after stabilization, there are still many cells at different heights

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microscopic imaging device and microscopic imaging method for accelerating cell sedimentation
  • Microscopic imaging device and microscopic imaging method for accelerating cell sedimentation
  • Microscopic imaging device and microscopic imaging method for accelerating cell sedimentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] The first embodiment of the present invention provides a microscopic imaging device for accelerating cell sedimentation, such as figure 1 shown, including:

[0075] An image acquisition chip, the image acquisition chip includes a photosensitive area 1 for collecting image information of a liquid-based cell sample;

[0076] At least one microfluidic chamber 2 is arranged on the photosensitive area 1, and the lower surface of the microfluidic chamber 2 is attached to the upper surface of the photosensitive area 1. The lower surface of the microfluidic chamber 2 is transparent, and the microfluidic chamber 2 for loading liquid-based cell samples;

[0077] Each microfluidic chamber 2 includes:

[0078] a hollow cavity 20;

[0079] Two through holes 21, the two through holes are symmetrically arranged on both sides of the microfluidic chamber 2, and communicate with the two ends of the hollow cavity 20 respectively, the position of the two through holes 21 is higher than ...

Embodiment 2

[0120] The second embodiment of the present invention provides a microscopic imaging device for liquid-based cell samples, such as figure 1 shown, including:

[0121] The photosensitive area 1 of an image acquisition chip is used to acquire image information of a liquid-based cell sample;

[0122] At least one microfluidic chamber 2 is arranged on the photosensitive area 1 of the image acquisition chip, and the lower surface of the microfluidic chamber 2 is attached to the upper surface of the photosensitive area 1 of the image acquisition chip, and the lower surface of the microfluidic chamber 2 It is transparent, and the microflow chamber 2 is used to load liquid-based cell samples;

[0123] Each microfluidic chamber 2 includes:

[0124] a hollow cavity 20;

[0125] Two through holes 21 are symmetrically arranged on both sides of the microfluidic chamber 2 and communicate with both ends of the hollow cavity 20 respectively. The positions of the two through holes 21 are high...

Embodiment 3

[0132] A third embodiment of the present invention provides a microscopic imaging device for liquid-based cell samples, such as Figure 16 shown, including:

[0133] A photosensitive area 1 of an image acquisition chip, used for acquiring image information of a liquid-based cell sample;

[0134] Two microfluidic chambers 2 are arranged on the photosensitive region 1, and the lower surface of each microfluidic chamber 2 is attached to the upper surface of the photosensitive region 1. The lower surface of the microfluidic chamber 2 is transparent, and the microfluidic chamber 2 is transparent. Chamber 2 is used to load liquid-based cell samples;

[0135] Each microfluidic chamber 2 includes:

[0136] a hollow cavity 20;

[0137] Two through holes 21 are symmetrically arranged on both sides of the microfluidic chamber 2 and communicate with both ends of the hollow cavity 20 respectively. The positions of the two through holes 21 are higher than the lower part of the microfluid...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
thicknessaaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a microscopic imaging device and a microscopic imaging method for accelerating cell sedimentation. The device comprises a photosensitive area of an image collection chip, at least one micro-flow chamber, two through holes and two conductive films, wherein the photosensitive region is used for collecting image information of a liquid-based cell sample; the at least one micro-flow chamber is arranged on the photosensitive area of the image collection chip, the lower surface of the micro-flow chamber is attached to the upper surface of the photosensitive area of the imagecollection chip, the lower surface of the micro-flow chamber is transparent, and the micro-flow chamber is used for loading the liquid-based cell sample; each micro-flow chamber includes a hollow cavity; the two through holes are symmetrically formed in the two sides of the micro-flow chamber; and the two conductive films cover the upper surface and the lower surface of the micro-flow chamber, aretransparent, and have opposite polarities. The technical scheme of the invention has the beneficial effects that the photosensitive area of the image collection chip is closer to the liquid-based cell sample so that the quality of a microscopic image is improved, and an electric field formed by the two conductive films can accelerate cell sedimentation.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a microscopic imaging device and a microscopic imaging method for accelerating cell sedimentation. Background technique [0002] The microscopic imaging device is used to optically magnify microbial tissues, and realize the detection and morphological analysis of liquid-based cell samples through the observation and recording of the photosensitive area of ​​the image acquisition chip. Traditional optical microscopes are limited by the contradiction between field of view and resolution, that is, the larger the magnification of microbial tissues, the more subtle images of microbial tissues can be seen, but at the same time, the field of view that can be observed will also be reduced. [0003] Most of the microscopic imaging devices in the prior art adopt the structure of the counting pool. For example, Chinese patent CN201520807546.8 discloses a microscope, which includ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N15/14B01L3/00
CPCB01L3/50273B01L3/502761B01L2200/0652B01L2300/0867B01L2400/0415G01N15/1404G01N21/6458G01N2015/1415G01N2021/6463
Inventor 杨程卢大骅
Owner 南京九川科学技术有限公司