Preparation method for and application of gene therapy vector

A technology of gene therapy and therapeutic effects, which is applied in the field of preparation of gene therapy vectors, can solve problems such as technical complexity, and achieve the effect of simple process, short process and improved production efficiency

Inactive Publication Date: 2021-01-05
杭州荣泽生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, germ cell gene therapy involves many problems and the technology is more complicated. Therefore, more somatic cell gene therapy is now used

Method used

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Embodiment Construction

[0024] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than Full examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] The invention provides a method for preparing a gene therapy carrier, which includes the steps of cell culture and stirring, virus inoculation, cell collection, ultrafiltration, centrifugation, HiTrop Q HP desalting, detection and the like:

[0026] A. The cell culture and stirring are as follows: the cells are cultured in the cell culture medium;

[0027] B. The virus inoculation is as follows: the tre...

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Abstract

The invention discloses a preparation method for a gene therapy vector. The preparation method comprises the following steps: cell culture and stirring, virus inoculation, cell collection, ultrafiltration, centrifugation, HiTrop Q HP desalination, detection and the like. The step of cell culture and stirring is to culture cells in a cell culture medium; the step of virus inoculation is to performinoculation of a therapy virus; the step of cell collection is to collect the cells after the number of the cells meets the need and detection is performed; the ultrafiltration step is to put the collected cells in an ultrafiltration tube for filtration; the step of centrifugation is to perform centrifugation through centrifugal equipment; the step of HiTrop Q HP is to recombine a virus vector byutilizing a method of anion exchange chromatography; and the the step of desalination is to perform desalination by utilizing a chromatographic column. According to the preparation method, the preparation efficiency can be remarkably improved by employing an anion exchange chromatography method to recombine the virus vector, and the preparation method has the characteristics of a simple process, short flow and low cost; gene therapy is carried out on hereditary diseases and acquired diseases of the human beings, and various refractory diseases of the human beings are cured.

Description

technical field [0001] The invention relates to the technical field of gene therapy carrier preparation, in particular to a preparation method and application of a gene therapy carrier. Background technique [0002] Gene therapy refers to the introduction of exogenous normal genes into target cells to correct or compensate for diseases caused by defects and abnormal genes, so as to achieve therapeutic purposes. It also includes technical applications such as genetic modification. That is, the exogenous gene is inserted into the appropriate recipient cells of the patient through gene transfer technology, so that the product produced by the exogenous gene can treat a certain disease. In a broad sense, gene therapy can also include measures and new technologies to treat certain diseases at the DNA level; [0003] Gene therapy for genetic diseases refers to the application of genetic engineering technology to introduce normal genes into patient cells to correct defective genes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867C12N15/861C12N15/864C12N15/869A61K48/00
CPCC12N15/86A61K48/0008C12N2740/10043C12N2740/15043C12N2710/10343C12N2750/14143C12N2710/16643
Inventor 陈相波雷鸣田朋飞应荣
Owner 杭州荣泽生物科技集团有限公司
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