Kit for detecting porcine pseudorabies virus (PRV) by combining centrifugal micro-fluidic chip with loop-mediated isothermal amplification (LAMP) technology

Abstract

The invention belongs to the technical field of molecular detection, discloses a kit for detecting porcine pseudorabies virus (PRV) by combining a centrifugal micro-fluidic chip with a loop-mediated isothermal amplification (LAMP) technology, and establishes a PRV nucleic acid detection method which is rapid, sensitive, high in accuracy and high in repeatability by combining the micro-fluidic chiptechnology. The micro-fluidic chip detection method is high in specificity, and the lowest detection limit can reach 10<2>copies/[mu]L. According to the micro-fluidic chip kit for detecting porcine pseudorabies virus (PRV), a chip analysis device is small and portable, and the defects of time consumption, labor consumption and high cost of PRV detection can be overcome, so that the detection sensitivity and specificity are improved, the labor and equipment cost is reduced, the detection period is shortened, and the requirements of field detection are met. The rapid detection technology can bepopularized and applied to epidemiological investigation and epidemic situation monitoring of the porcine pseudorabies virus (PRV), and has good practical significance and wide market prospects.

Description

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AI Technical Summary

Benefits of technology

This patented describes an improved way to diagnose Porcoidum parvovirus (PPR) disease caused by bacteria called Pseudoraimus or Pasteurium spp., through testing different sequence types from genome DNA found within these organisms. By doing that we have developed a simpler but more reliable process than previous methods such as PCR. Overall, our new approach provides faster and better ways to identify diseases associated with Psoriasis without requiring expensive specialized tests like ELISA.

Problems solved by technology

This patented describes different techniques for identifying and measuring PPRV antibodies in order to prevent veterinary issues such as severe respiratory syndrome associated with swines due to this type of virus. However current technologies require expensive instruments like electronics and advanced analyzer systems, making their practicality limited at home settings where they may lack accessibility. There exists a new method for quick identification of PPRvims while reducing costs compared to existing methods.

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