Shaddock pulp aqueous extract powder as well as preparation, ischemic stroke treatment effect and application thereof

A technology of water extract and grapefruit meat, applied in the field of biomedicine, can solve the problems of no curative effect, thoracic cavity and abdominal bleeding, etc.

Inactive Publication Date: 2021-01-15
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two insurmountable problems in the treatment of ischemic stroke with rtPA
The first complication is that rtPA is not effective in patients with ischemic stroke >4 hours
The second problem is that continuous use of rtPA can cause cerebral, thoracic and abdominal hemorrhage

Method used

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  • Shaddock pulp aqueous extract powder as well as preparation, ischemic stroke treatment effect and application thereof
  • Shaddock pulp aqueous extract powder as well as preparation, ischemic stroke treatment effect and application thereof
  • Shaddock pulp aqueous extract powder as well as preparation, ischemic stroke treatment effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 prepares pomelo meat water extract powder

[0041] Wash the fresh pomelo meat with tap water, and chop 100g of fresh pomelo meat. Heat in 500 mL of distilled water at 50°C-80°C for 2 hours while stirring (220 rpm / min). Cool to room temperature, filter, and concentrate the filtrate under reduced pressure to obtain 30 g of fresh pomelo meat water extract powder. It is fresh pomelo meat water extract powder.

Embodiment 2

[0042] Example 2 Determination of the chromatogram and mass spectrometry ion chromatogram of pomelo meat aqueous extract powder

[0043] 2-1. Preparation of sample solution (10mg / mL)

[0044] Weigh 26.7mg of pomelo pulp aqueous extract powder, and dissolve the powder in 2.67mL of ultrapure water. The obtained solution was ultrasonically shaken for 1 minute, and then centrifuged at 13000r / min for 10 minutes. Take the supernatant, pass through a 0.22μm filter membrane, and place it in a sample bottle for chromatography and mass spectrometry.

[0045] 2-2. Chromatographic conditions

[0046] Column: Waters, Acquity HSS T3 column (2.1×100mm i.d., 1.7μm); injection volume: 2μL; PDA detector: 190-400nm; mobile phase: water (0.1% formic acid), acetonitrile; use this mobile phase and follow the gradient in Table 1 Wash the column.

[0047] Table 1 Mobile phase gradient

[0048]

[0049] 2-3. Conditions for measuring ion current

[0050] Electrospray ionization mode: positi...

Embodiment 3

[0053] Embodiment 3 specifies the structure of 25 components in the pomelo meat aqueous extract powder

[0054] The UPLC chromatography of Example 2 was coupled with mass spectrometry, and the UPLC-mass spectrometry of pomelo meat water extract powder was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive ion and negative ion. The slope well collision energy is 20-60V. The ion source temperature was 120°C. The pressure of the nebulizer is 6.5 bar. The mass / charge ratio ranges from 100-1500. 25 independent peaks resolved within 47 minutes. According to the fragmentation law of mass spectrometry, these peaks (the peaks of the total ion current spectrum are numbered sequentially from left to right) are designated as the corresponding components in Table 2. The cleavage products of these 25 components under mass spectrometry conditions are shown in Figure 2-26 .

[0055] Table 2 Retention time, mass number, structure and name of...

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PUM

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Abstract

The invention discloses fresh shaddock pulp aqueous extract powder, a preparation method of the fresh shaddock pulp aqueous extract powder and an application of the fresh shaddock pulp aqueous extractpowder in thrombolysis medicines and medicines still effective for treating ischemic stroke which breaks out for 24 hours. The fresh shaddock pulp aqueous extract powder prepared by the method provided by the invention contains 15 quinic acid compounds and 10 flavonoid compounds. Experiments prove that the fresh shaddock pulp aqueous extract powder not only has a good thrombolysis effect, but also has an exact curative effect on mice suffering from the ischemic stroke which breaks out for 24 hours, so that a technical means is provided for thrombolysis treatment, especially for effective treatment of the ischemic stroke which breaks out for 24 hours.

Description

technical field [0001] The present invention relates to a method for preparing water extract powder of fresh pomelo meat, and relates to the application of the water extract powder of fresh pomelo meat prepared by the method in the preparation of thrombolytic drugs and the preparation of drugs that are still effective for ischemic stroke for 24 hours application in medicine. The invention belongs to the field of biomedicine. Background technique [0002] Among the morbidity and mortality of cardiovascular diseases, the morbidity and mortality of acute pulmonary embolism ranks third. If left untreated, approximately 30% of patients die. From the perspective of the pathogenesis process, the blood clot formed in the deep vein leaves the deep vein through the venous system, passes through the right ventricle and enters the pulmonary artery to form pulmonary embolism. Pulmonary parenchymal necrosis can result due to deterioration of arterial circulation. If treated, approxima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61K31/225A61K31/222A61K31/7048A61P9/10A61P7/02A61K131/00
CPCA61K31/222A61K31/225A61K31/7048A61K36/752A61K2236/331A61K2236/39A61P7/02A61P9/10
Inventor 彭师奇赵明黄飞
Owner 北京恒润泰生医药科技有限公司
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