Method for establishing efficient regeneration system of chrysanthemum morifolium ramat and application thereof
A high-efficiency regeneration technology of Chrysanthemum chrysanthemum, applied in the field of plant propagation, can solve the problems of trait degradation, long seedling cultivation cycle, low reproduction coefficient, etc., and achieve the effect of low production cost, long seedling cultivation cycle and high reproduction coefficient
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Embodiment 1
[0056] A kind of method that establishes the efficient regeneration system of Chrysanthemum chrysanthemum, with reference to Figure 1-3 as shown ( figure 1 In order to provide a schematic diagram of the method for establishing a high-efficiency regeneration system of Chrysanthemum chrysanthemum at the time of inducing culture initiation, figure 2 In order to provide a schematic diagram of the method for establishing a high-efficiency regeneration system of Chrysanthemum chrysanthemum during proliferation and culture, image 3 The schematic diagram of the method for establishing the high-efficiency regeneration system of Chrysanthemum chrysanthemum during rooting culture) provided, the method for establishing the high-efficiency regeneration system of Chrysanthemum chrysanthemum specifically includes the following steps:
[0057] 1) taking the explant of the female parent of Chrysanthemum chrysanthemum to obtain the explant material;
[0058] 2) Inducing culture of the expl...
Embodiment 2
[0062] A kind of method that establishes the efficient regeneration system of Chrysanthemum chrysanthemum, with reference to Figure 1-3 as shown ( figure 1 In order to provide a schematic diagram of the method for establishing a high-efficiency regeneration system of Chrysanthemum chrysanthemum at the time of inducing culture initiation, figure 2 In order to provide a schematic diagram of the method for establishing a high-efficiency regeneration system of Chrysanthemum chrysanthemum during proliferation and culture, image 3 The schematic diagram of the method for the establishment of the high-efficiency regeneration system of Chrysanthemum chrysanthemum when rooted) for the establishment of the provided system, specifically comprises the following steps:
[0063] 1. Maternal explant pretreatment
[0064] The female parent of Chrysanthemum chrysanthemum was planted in a greenhouse to accelerate germination, sprayed with 0.1% carbendazim once a week, and sprayed three times ...
Embodiment 3
[0087] Compared with embodiment 2, except that the pH value of rooting medium is 5.8, other is identical with embodiment 2.
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