Micro-fluidic chip and cell screening device and method
A microfluidic chip and screening device technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of easy missed detection of tumor cells, low detection efficiency, and individual heterogeneity advanced questions
Active Publication Date: 2021-01-29
上海汉原生物科技有限公司
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Problems solved by technology
However, this method has a large defect: due to the high individual heterogeneity of CTCs, the antibody-based immunoaffinity capture method can only capture epithelial tumor cells, which has a high false negative rate and is easy to miss detection of other different sources. Tumor cells, such as mesenchymal-derived, stem cell-derived and other tumor cells
However, the disadvantage of the existing physical characteristic screening method is that the device capture effect is poor, resulting in low detection efficiency
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[0061] The process of cell screening using microfluidic chip 1 is as follows: figure 1 shown. A cell screening device 100 includes a sampling pump set 2 , a microfluidic chip 1 , and a waste liquid collection unit 3 . The sampling pump group 2 is used to pump the liquid to the microfluidic chip 1 , and the waste liquid collecting part 3 is used to collect the waste liquid discharged from the microfluidic chip 1 . The injection pump group 2 delivers the preset liquid to the microfluidic chip 1 through the reversing valve 6, and the preset liquid includes but is not limited to figure 1 type shown.
[0062] The injection pump group 2 first pumps a surface treatment solution P, such as polyvinylpyrrolidone, into the microfluidic chip 1 to perform surface treatment on the chip, and then pumps a buffer solution M, such as phosphoric acid, into the surface-treated microfluidic chip 1. Salt buffer to fill each fluid channel in the chip and eliminate air bubbles in the microchannel....
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Abstract
The invention discloses a micro-fluidic chip which comprises a screening pipeline, an inlet channel and an outlet channel which are arranged in parallel are provided in the screening pipeline, the inlet channel and the outlet channel are separated by a separation part, and the separation part is provided with a screening unit. The screening unit is communicated with the inlet channel and the outlet channel, a screening pipeline comprises a turning section, the centrifugal side of the inlet channel in the turning section is provided with the screening unit communicated with the outlet channel,and the micro-fluidic chip can achieve efficient interception of target cells. The invention further discloses a cell screening device and a cell screening method using the micro-fluidic chip.
Description
technical field [0001] The present disclosure relates to the field of microfluidic chips, in particular to the use of microfluidic chips for cell screening and capture. Background technique [0002] In various biological detection scenarios, it is necessary to use a cell screening device to separate target cells from blood, so as to perform further operations on the target cells, such as observation or inspection. For example, the nervous and immune functions of the body may change under conditions such as radiation exposure. At this time, it is necessary to detect the status of immune cells (Immune Cells) at different stages, and for example, circulating tumors that enter the peripheral blood of the human body during the growth of solid tumors Cells (CTC, Circulating Tumor Cells), under suitable conditions, these cells will further multiply to form secondary tumors, which seriously affects the development of the patient's condition. Therefore, it is necessary to separate th...
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IPC IPC(8): B01L3/00C12M1/00
CPCB01L3/5027C12M47/04
Inventor 杨旸庄紫云姜奥
Owner 上海汉原生物科技有限公司